Myeloid Leukemia

Table 1
Selected Microsatellite Loci
CHLC Chrom. PCR products
Locus Marker accession location Type Het Alleles lenghth (in bp) Primer sequences 5�-3�
1 D3S3045 GATA84B12 32627 3 TetNR 0,82 7 176–208 Forw.: ACCAAATGAGACAGTGGCAT
Rev.: ATGAGGACGGTTGACATCTG
2 D4S2366 GATA22G05 31823 4 TetNR 0,79 7 120–144 Forw.: TCCTGACATTCCTAGGGTGA
Rev.: AAAACAAATATGGCTCTATCTATCG
3 D12S1064 GATA63D12 40934 12 TetNR 0,82 8 173–201 Forw.: ACTACTCCAAGGTTCCAGCC
Rev.: AATATTGACTTTCTCTTGCTACCC
4 D16S539 GATA11C06 715 16 TetNR 0,76 12 148–172 Forw.: GATCCCAAGCTCTTCCTCTT
Rev.: ACGTTTGTGTGTGCATCTGT
5 D17S1290 GATA49C09 40873 17 TetNR 0,84 9 170–210 Forw.: GCCAACAGAGCAAGACTGTC
Rev.: CGAAACAGTTAAATGGCCAA
The most informative set of short tandem repeat (STR) loci from the authors’ laboratory, which has provided an adequate marker in the majority of all
donor/recipient constellations tested. A series of successful marker sets from different European laboratories has been recently published (6–11). Abbreviations:
Chrom = chromosomal; TetNR = tetranucleotide repeat marker; Het = heterozygosity. For the selection of appropriate markers for initial
genotyping and subsequent analysis of chimerism (see also Notes 21–24, 28).
280 Lion and Watzinger