Myeloid Leukemia

32 Picard, Silvy, and Gabert
Fig. 2. Double-stranded DNA detection with SYBR Green I dye incorporation.
During denaturation, unbound SYBR Green I dye exhibits little fluorescence. At the
annealing temperature, a few dye molecules bind to the primer-target double strand,
resulting in light emission upon excitation. During the polymerization step, more and
more dye molecules bind to the newly synthesized DNA and the increasing fluorescence
can be monitored. On denaturation, the dye molecules are released and the fluorescence
signal returns to background.
tinct peaks when plotting the first negative derivative of fluorescence vs temperature.
If only the specific PCR product has been formed, a single peak
should be visible in the melting peak profile.