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146 Mokany et al.<br />

21. It is possible to set looser acceptance criteria if the investigator realizes that this<br />

limits the discrimination of the assay, for example a ratio between 0.33 and 3<br />

indicates that the assay cannot discriminate differences less than threefold.<br />

References<br />

1. Grimwade, D. and Enver, T. (2004) Acute promyelocytic leukemia: where does it<br />

stem from? <strong>Leukemia</strong> 18(3), 375–384.<br />

2. Mistry, A. R., Pedersen, E. W., Solomon, E., and Grimwade, D. (2003) The molecular<br />

pathogenesis of acute promyelocytic leukaemia: implications for the clinical<br />

management of the disease. Blood Reviews 17(2), 71–91.<br />

3. Degos, L. and Wang, Z. Y. (2001) All trans retinoic acid in acute promyelocytic<br />

leukemia. Oncogene 20, 7140–7145.<br />

4. Grimwade, D. and Enver, T. (2002) The significance of minimal residual disease<br />

in patients with t(15;17). Best Pract. Res. Clin. Haematol. 15(1), 137–158.<br />

5. Lo Coco, F., Diverio, D., Avvisati, G., et al. (1999) Therapy of molecular relapse<br />

in acute promyelocytic leukemia. Blood 94, 2225–2229.<br />

6. Grimwade, D. and Lo Coco, F. (2002) Acute promyelocytic leukemia: a model<br />

for the role of molecular diagnosis and residual disease monitoring in directing<br />

treatment approach in acute myeloid leukemia. <strong>Leukemia</strong> 16, 1959–1973.<br />

7. Applegate, T. L., Iland, H. J., Mokany, E., and Todd, A. V. (2002) Diagnosis and<br />

molecular monitoring of acute promyelocytic leukemia using DzyNA reverse transcription-PCR<br />

to Quantify PML-RARα fusion transcripts. Clin. Chem. 48(8),<br />

1338–1343.<br />

8. Applegate, T.L., Iland, H. J., Mokany, E., and Todd, A. V. (2002) Molecular monitoring<br />

of acute promyelocytic leukemia by DzyNA reverse transcriptase. Clinical<br />

Chemistry 48, 1858–1860.9. Jurcic, J. G. (2003) Monitoring PML-RARalpha in<br />

acute promyelocytic leukemia. Curr. Oncol. Rep. 5(5), 391–398.<br />

9. Jurcic, J. G. (2003) Monitoring PML-RARalpha in acute promyelocytic leukemia.<br />

Curr. Oncol. Rep. 5, 391–398.<br />

10. Gu, B. W., Hu, J., and Xu, L. (2001) Feasibility and clinical significance of realtime<br />

quantitative RT-PCR assay of PML-RARalpha fusion transcript in patients<br />

with acute promyelocytic leukemia. Hematol. J. 2(5), 330–340.<br />

11. Slack, J. L., Bi, W., and Livak, K. J. (2001) Pre-clinical validation of a novel,<br />

highly sensitive assay to detect PML-RARalpha mRNA using real-time reversetranscription<br />

polymerase chain reaction. J. Mol. Diagn. 3(4), 141–149.<br />

12. Gabert, J. and Beillard, E. (2003) Standardization and quality control studies of<br />

’real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion<br />

gene transcripts for residual disease detection in leukemia—a Europe Against<br />

Cancer program. <strong>Leukemia</strong> 17(12), 2318–2357.<br />

13. Larsen, R. (2003) BD QZyme Assays for Quantitative PCR. Clontechniques<br />

XVIII(4), 2–3.<br />

14. Larsen, R., Sheng, D., and Tan, M. (2004) Successful Duplexing of Randomly<br />

Selected BD QZyme qPCR Assays. Clontechniques XIX, 10–11.

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