Myeloid Leukemia

Classification of AML by DNA-Oligonucleotide Microarrays 235
Fig. 6. Classification task. The support vector machine separates a given set of binary
labeled training data with a hyperplane that is maximally distant from them (maximal
margin). The middle black line is the decision surface defining the borderline between
the area of prediction of type I samples (squares) and type II samples (circles). The outer
lines precisely meet the constraint. Support vectors marked as critical for the classification
task are the points that lie closest to the separating hyperplane.
3.4.6. Functional Gene Annotation: NetAffx Database
The NetAffx database can be used to functionally annotate the probe sets
represented on the microarrays. The NetAffx Analysis Center
(www.affymetrix.com/analysis/) is an integrated, freely available online
resource created by Affymetrix (21). This Web-based application enables
researchers to correlate results from experiments with array design and annotation
information. It is a dynamic tool and provides access to underlying array
content and the design of GeneChip probe arrays, including probe sequences
and extensive gene annotations from both Affymetrix and the public domain. It
allows the user to search array contents for sequences of interest, review gene
and protein characterizations for represented probe sets, and sort transcripts by
functional group, metabolic pathway, and disease association. For each cataloged
Affymetrix GeneChip microarray, an anchoring databank summarizes
all the annotations for the probe sets. The information provided for each probe
set falls into two categories: sequence annotations and static information.
Sequence annotations refer to the information about the representative
sequence for a probe set, including functional annotations for gene title, gene