Myeloid Leukemia

174 van der Reijden and Jansen
10. The cDNA quality is determined as follows: the ∆Ct of the reference gene between
a sample and the laboratory standard value should be less than 2. The
laboratory standard value is obtained by using the mean Ct of over 100 samples
with fixed input (50 ng cDNA).
11. As a standard, we have used the CBFB-MYH11-positive cell line ME-1 (13).
Alternatively, a CBFB-MYH11-positive patient sample can be used. The smallest
difference in MYH11 expression between the CBFB-MYH11-positive patient with
the lowest MYH11 expression and the CBFB-MYH11-negative patient with the
highest MYH11 expression was 25-fold (13).
References
1. Liu, P. P., Hajra, A., Wijmenga, C., and Collins, F. S. (1995) Molecular pathogenesis
of the chromosome 16 inversion in the M4Eo subtype of acute myeloid leukemia.
Blood 85, 2289–2302.
2. Langabeer, S. E., Walker, H., Gale, R. E., et al. (1997) Frequency of CBF beta/
MYH11 fusion transcripts in patients entered into the U.K. MRC AML trials. The
MRC Adult Leukaemia Working Party. Br. J. Haematol. 96, 736–739.
3. van der Reijden, B. A., Simons, A., Luiten, E., et al. (2002) Minimal residual
disease quantification in patients with acute myeloid leukaemia and inv(16)/
CBFB-MYH11 gene fusion. Br. J. Haematol. 118, 411–418.
4. Guerrasio, A., Pilatrino, C., De Micheli, D., et al. (2002) Assessment of minimal
residual disease (MRD) in CBFbeta/MYH11-positive acute myeloid leukemias
by qualitative and quantitative RT-PCR amplification of fusion transcripts. Leukemia
16, 1176–1181.
5. Claxton, D. F., Liu, P., Hsu, H. B., et al. (1994) Detection of fusion transcripts
generated by the inversion 16 chromosome in acute myelogenous leukemia. Blood
83, 1750–1756.
6. Poirel, H., Radford-Weiss, I., Rack, K., et al. (1995) Detection of the chromosome
16 CBF beta-MYH11 fusion transcript in myelomonocytic leukemias. Blood
85, 1313–1322.
7. Hebert, J., Cayuela, J. M., Daniel, M. T., Berger, R., and Sigaux, F. (1994) Detection
of minimal residual disease in acute myelomonocytic leukemia with abnormal
marrow eosinophils by nested polymerase chain reaction with allele specific
amplification. Blood 84, 2291–2296.
8. van der Reijden, B. A., Lombardo, M., Dauwerse, H. G., et al. (1995) RT-PCR
diagnosis of patients with acute nonlymphocytic leukemia and inv(16)(p13q22)
and identification of new alternative splicing in CBFB-MYH11 transcripts. Blood
86, 277–282.
9. van der Reijden, B. A., de Wit, L., van Der Poel, S., et al. (2001) Identification of
a novel CBFB-MYH11 transcript: implications for RT- PCR diagnosis. Hematol.
J. 2, 206–209.
10. Springall, F. H., Lukeis, R. L., Tyrrell, V., Joshua, D. E., and Iland, H. J. (1998)
Identification of a novel CBFB-MYH11 fusion transcript in a patient with AML
and inversion of chromosome 16. Leukemia 12, 2034–2035.