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Acknowledgements Book of abstracts - Publicaties - Vlaanderen.be

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Pol Llonch presents Poster 65 during the poster sessions in the Aula.<br />

Session theme 6: Free topics<br />

137<br />

Poster 65<br />

AVERSION TO THE INHALATION OF NITROGEN AND CARBON<br />

DIOXIDE MIXTURES USED FOR STUNNING IN PIGS<br />

P. Llonch 1 , A. Dalmau 1 , P. Rodríguez 1 , J.L. Ruiz de la Torre 2 , X. Manteca 2 , A. Velarde 1<br />

1 Finca Camps i Armet s/n. 17121 Monells, Girona, Spain<br />

2 School <strong>of</strong> Veterinary Science, Edifici V, Campus Unisersitari UAB, 08193, Cerdanyola del Vallès,<br />

Barcelona, Spain<br />

In the CO2 stunning system, pigs are loaded into a crate which is then lowered into a pit prefilled<br />

with high concentration <strong>of</strong> CO2. However, the inhalation <strong>of</strong> concentrations above 30% CO2 by<br />

volume in atmospheric air causes aversion in pigs. The objective <strong>of</strong> the study was to assess the<br />

aversiveness <strong>of</strong> alternative N2 and CO2 (up to 30% by volume in atmospheric air) mixtures to stun<br />

pigs<br />

The aversion to the inhalation <strong>of</strong> 85% nitrogen and 15% CO2 (85N15C), 80% nitrogen and<br />

20%CO2 (80N20C), or 70% nitrogen and 30% CO2 (70N30C) were assessed in 3 different trials <strong>of</strong><br />

10 pigs each, using aversion learning test and <strong>be</strong>havioural studies. Pigs entered the crate<br />

individually and descended into the pit during 6 consecutive sessions. During the first 3 sessions the<br />

pit contained atmospheric air and the following 3 sessions it contained the gas mixture. The<br />

following <strong>be</strong>havioural parameters were recorded: time to cross the raceway and enter the crate,<br />

presence and time taken to perform the first retreat attempt, escape attempts, gasping as sign <strong>of</strong><br />

breathlessness and loss <strong>of</strong> posture as sign <strong>of</strong> unconsciousness. Proc MIXED and Proc GENMOD<br />

statements <strong>of</strong> SAS were used for the variable “time to cross the raceway and enter the crate” and for<br />

the ordinal data, respectively.<br />

After the training sessions, pigs were well adapted to the facilities. For 85N15C and 70N30C<br />

groups, the time taken to cross the raceway and enter the crate increased after the first exposure to<br />

the gas (p0.05) were found <strong>be</strong>tween treatments on<br />

the aversive <strong>be</strong>haviour into the crate, pigs took less time (p

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