John M. S. Bartlett.pdf - Bio-Nica.info

Table 1
Oligonucleotides
Description Size (nt) Modifications Sequence *,†
SNP 7 amplicon 188 None GTCCCAAGCT GCATGATTGC TCTTTCTCCT TCTTCCCTGA GTCTCTCTCC
ATGCCCCTCA TCTCTTCCTT TTGCCCTCGC CTCTTCCATC CAYGTCTTCC
AAGGCCTGAT GCATTCATAA GTTGAAGCCC TCCCCAGATC CCCTTGGAGC
CTCTGCCCTC CTCCAGCCCG GATGGCTCTC CTCCATTT
Forward PCR primer for SNP 7 120 None GTC CCA AGC TGC ATG ATT GC
Reverse PCR primer for SNP 7 120 None AAA TGG AGG AGA GCC ATC CG
cZipCode 14 with 20 nt Luciferase tag 145 5′ amino, C15 spacer CAG GCC AAG TAA CTT CTT CGG GAT TGC ACC GTC AGC ACC
ACC GAG
Complement to Luciferase tag 120 5′ biotin or FITC CGA AGA AGT TAC TTG GCC TG
OLA reporter for SNP 7 114 5′ PO4, 3′ FITC GTC TTC CAA GGC CT
OLA capture probe for C allele with 150 None CTC GGT GGT GCT GAC GGT GCA ATC CTT TTG CCC TCG CCT
ZipCode 14 CTT CCA TCC Ac
OLA capture probe for T allele with 150 None CTC GGT GGT GCT GAC GGT GCA ATC CTT TTG CCC TCG CCT
ZipCode 14 CTT CCA TCC At
SBCE capture probe with ZipCode 14 149 None CTC GGT GGT GCT GAC GGT GCA ATC CTT TTG CCC TCG CCT
CTT CCA TCC A
* All sequences are written 5′ to 3′.
† The polymorphic base at the 3′ end of the OLA capture probe sequence is shown in lower case.
126 Iannone et al.