John M. S. Bartlett.pdf - Bio-Nica.info

Detection of MIS 307
Fig. 2. The top graph shows LOH, as the peak at 104 base pairs is missing. However, the
bottom graph demonstrates retention of both alleles.
of the two alleles are assigned to the peaks of greatest height and the smaller peaks are
interpreted as stutter bands.
3. Tumor cells are not completely separated from adjacent normal cells by microdissection.
Therefore, a major advantage of the fluorescence-based method is that the loss of alleles
can be determined precisely by calculation of the ratio of the peak heights of normal
and tumor alleles.
4. Assays of samples with allele ratios in the border line range of 0.6 to 0.7 were repeated at
least three times to ensure accuracy of results. Most repeated assays gave consistent results,
and all informative markers gave similar allele ratios in the same sample.
References
1. King, B. L., Carcangiu, M.-L., Carter, D., Kiechle, M., Pfisterer, J., and Kaoinski, B. M.
(1995) Microsatellite instability in ovarian neoplasms. Br. J. Cancer 72, 376–382.
2. Sardi, I., Bartoletti, R., Occhini, I., Piazzini, M., Travaglini, F., Guazzelli, R., et al. (1999)
Microsatellite alterations in superficial and locally advanced transitional cell carcinoma of
the bladder. Oncol. Reports 6, 901–905.