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24 Stirling
Page 1 and 2: TM Methods in Molecular Biology Vol
Page 3 and 4: Xin Wang and W. Scott Young III 105
Page 5 and 6: 63. Primed In Situ Nucleic Acid Lab
Page 7 and 8: 4 Bartlett and Stirling The thing t
Page 9 and 10: 6 Bartlett and Stirling Fig. 2. Res
Page 11 and 12: 8 Carroll and Casimir of PCR. Such
Page 13 and 14: 10 Carroll and Casimir cost more th
Page 15 and 16: 12 Carroll and Casimir are no longe
Page 17 and 18: 14 Carroll and Casimir Should these
Page 19 and 20: 16 McDonagh Fig. 1. Unidirectional
Page 21 and 22: 18 McDonagh stored. This means that
Page 23 and 24: 20 McDonagh
Page 25: 22 Stirling which will either not a
Page 29 and 30: 28 Bartlett References 1. US Depart
Page 31 and 32: 30 Bartlett and White 11. 5 M sodiu
Page 33 and 34: 32 Bartlett and White
Page 35 and 36: 34 Pearson and Stirling 11. Microfu
Page 37 and 38: 36 Going 3. Methods 3.1. Section Cu
Page 39 and 40: 38 Going pipet filler. Spread the p
Page 41 and 42: 40 Going digitally to record the di
Page 43 and 44: 42 Going
Page 45 and 46: 44 Pearson 7. Add 60 µL of chlorof
Page 47 and 48: 46 Bartlett 3. Methods 3.1. RNA Ext
Page 49 and 50: 48 Pearson 3. Method The method of
Page 51 and 52: 50 Stirling and Bartlett 4. Protein
Page 53 and 54: 52 Stirling and Bartlett
Page 55 and 56: 54 Stirling 3. Methods 3.1. Fungal
Page 57 and 58: 56 McDonagh 2. Add 0.4 mL of TNE bu
Page 59 and 60: 58 Schmerer 2. Materials To apply t
Page 61 and 62: 60 Schmerer 3. The additional purif
Page 63 and 64: 62 Schmerer
Page 65 and 66: 64 Stirling
Page 67 and 68: 66 Bartlett Table 1 Recommended Aga
Page 69 and 70: 68 Bartlett Table 2 Separation of D
Page 71 and 72: 70 Bartlett 2. 5× TBE: 54 g of Tri
Page 73 and 74: 72 Bartlett 7. Remove upper aqueous
Page 75 and 76: 74 Bartlett 4. Many modern electrop
Page 77 and 78:
76 Bartlett
Page 79 and 80:
78 Stirling 11. Store at -20°C for
Page 81 and 82:
82 Hyndman and Mitsuhashi 3.1. Effi
Page 83 and 84:
84 Hyndman and Mitsuhashi Fig. 1. H
Page 85 and 86:
86 Hyndman and Mitsuhashi Fig. 3. H
Page 87 and 88:
88 Hyndman and Mitsuhashi can be ge
Page 89 and 90:
90 Grunenwald may be affected by ea
Page 91 and 92:
92 Grunenwald 50°C will generally
Page 93 and 94:
94 Grunenwald of template DNA, a su
Page 95 and 96:
96 Grunenwald than absolutely neces
Page 97 and 98:
98 Grunenwald 2. Foord, O. S. and R
Page 99 and 100:
100 Grunenwald
Page 101 and 102:
102 Stirling Table 1 Thermal Cycler
Page 103 and 104:
104 Stirling
Page 105 and 106:
106 Wang and Young full-length cDNA
Page 107 and 108:
108 Wang and Young Fig. 1. (A) Nort
Page 109 and 110:
110 Wang and Young Fig. 3. Expresse
Page 111 and 112:
112 Wang and Young 2. First-strand
Page 113 and 114:
114 Wang and Young 3′-RACE outlin
Page 115 and 116:
116 Wang and Young
Page 117 and 118:
118 Dassanayake and Samaranayake co
Page 119 and 120:
120 Dassanayake and Samaranayake 5.
Page 121 and 122:
122 Dassanayake and Samaranayake Re
Page 123 and 124:
124 Iannone et al. Fig. 1. Diagram
Page 125 and 126:
Table 1 Oligonucleotides Descriptio
Page 127 and 128:
128 Iannone et al. 5. For microsphe
Page 129 and 130:
130 Iannone et al. 4. To adjust for
Page 131 and 132:
132 Iannone et al. 6. Target concen
Page 133 and 134:
134 Iannone et al.
Page 135 and 136:
136 Benjamin, Smith, and Waites amp
Page 137 and 138:
138 Benjamin, Smith, and Waites the
Page 139 and 140:
140 Benjamin, Smith, and Waites 2.2
Page 141 and 142:
142 Benjamin, Smith, and Waites 2.
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
148 Benjamin, Smith, and Waites 8.
Page 149 and 150:
150 Benjamin, Smith, and Waites
Page 151 and 152:
152 Olmos et al. Fig. 1. RT-hemines
Page 153 and 154:
154 Olmos et al. This nested RT-PCR
Page 155 and 156:
156 Olmos et al. Table 2 Volume and
Page 157 and 158:
158 Olmos et al. 8. The sensitivity
Page 159 and 160:
160 Olmos et al.
Page 161 and 162:
162 Abe 5. Moloney murine leukemia
Page 163 and 164:
164 Abe Table 2 Detection Rate of H
Page 165 and 166:
166 Abe 4. For HBV, nested PCR usin
Page 167 and 168:
168 Tellier et al. of Pfu (and ther
Page 169 and 170:
170 Tellier et al. 2. Cline, J., Br
Page 171 and 172:
172 Tellier et al. 38. Tamiya, S.,
Page 173 and 174:
174 Tellier et al. 13. Thin-wall PC
Page 175 and 176:
176 Tellier et al. 13 min for the l
Page 177 and 178:
178 Tellier et al.
Page 179 and 180:
182 Stirling efficiency of the reac
Page 181 and 182:
184 Stirling
Page 183 and 184:
186 Cremer and Moos Fig. 1. Rearran
Page 185 and 186:
188 Cremer and Moos 4. Count cells
Page 187 and 188:
190 Cremer and Moos Fig. 3. Alignme
Page 189 and 190:
192 Cremer and Moos Fig. 4. Testing
Page 191 and 192:
194 Cremer and Moos 5. Compare the
Page 193 and 194:
196 Cremer and Moos 11. Klein, E.,
Page 195 and 196:
198 McDonagh the dilution method is
Page 197 and 198:
200 McDonagh 2.2. Basic PCR 1. dNTP
Page 199 and 200:
202 McDonagh Fig. 2. Quantitative P
Page 201 and 202:
204 McDonagh
Page 203 and 204:
206 Bartlett Table 1 Example Assay
Page 205 and 206:
208 Bartlett 3.4. Calculation of Re
Page 207 and 208:
210 Bartlett 11. Cerenkov counting
Page 209 and 210:
212 Kerr Fig. 1. Fluorescent probes
Page 211 and 212:
214 Kerr after the PCR. This reduce
Page 213 and 214:
218 Bartlett As with any experiment
Page 215 and 216:
220 Bartlett Even once novel regula
Page 217 and 218:
222 Bartlett Notwithstanding these
Page 219 and 220:
224 Bartlett
Page 221 and 222:
226 Dominguez et al. Table 1 Primer
Page 223 and 224:
228 Dominguez et al. 4. Oligonucleo
Page 225 and 226:
230 Dominguez et al. Fig. 2. cDNA n
Page 227 and 228:
232 Dominguez et al. 3.4. Gel Elect
Page 229 and 230:
234 Dominguez et al. 3. If the cont
Page 231 and 232:
236 Dominguez et al. 11. Joshi, C.
Page 233 and 234:
238 Khalturin, Kuznetsov, and Bosch
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
246 Ringquist et al. In this chapte
Page 243 and 244:
248 Ringquist et al. 5. Total RNA s
Page 245 and 246:
250 Ringquist et al. 3. Purificatio
Page 247 and 248:
252 Ringquist et al. 2. The present
Page 249 and 250:
254 Ringquist et al.
Page 251 and 252:
256 Case-Green, Pritchard, and Sout
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Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Page 267 and 268:
272 Oien Fig. 1. A schematic diagra
Page 269 and 270:
274 Oien 4. 100% and 70% ethanol. 5
Page 271 and 272:
276 Oien 2. Purify polyA + mRNA fro
Page 273 and 274:
278 Oien 50-mL conical tubes. Resus
Page 275 and 276:
280 Oien Forward Primer, and then r
Page 277 and 278:
282 Oien 8. PCR. With SAGE, achievi
Page 279 and 280:
284 Oien
Page 281 and 282:
288 Edwards and Bartlett technology
Page 283 and 284:
290 Edwards and Bartlett ing less p
Page 285 and 286:
292 Edwards and Bartlett Stepwise m
Page 287 and 288:
294 Edwards and Bartlett
Page 289 and 290:
296 Rithidech and Dunn 11. Ethidium
Page 291 and 292:
298 Rithidech and Dunn Fig. 1. PCR
Page 293 and 294:
300 Rithidech and Dunn
Page 295 and 296:
302 Edwards and Bartlett Studies th
Page 297 and 298:
304 Edwards and Bartlett 11. Hot st
Page 299 and 300:
306 Edwards and Bartlett Fig. 1. Ex
Page 301 and 302:
308 Edwards and Bartlett 3. Sartor,
Page 303 and 304:
310 Schmerer the investigation conc
Page 305 and 306:
312 Schmerer References 1. Kunkel,
Page 307 and 308:
314 Schmerer
Page 309 and 310:
316 Aubele and Smida 2.2. Chemicals
Page 311 and 312:
318 Aubele and Smida References 1.
Page 313 and 314:
320 Nakashima, Akahoshi, and Tanaka
Page 315 and 316:
322 Nakashima, Akahoshi, and Tanaka
Page 317 and 318:
324 Stirling 9. TAE (20×): 484 g o
Page 319 and 320:
326 Stirling
Page 321 and 322:
328 Han and Robinson Fig. 1. Basic
Page 323 and 324:
330 Han and Robinson sequence diffe
Page 325 and 326:
332 Han and Robinson 4. Notes 1. PC
Page 327 and 328:
334 Han and Robinson
Page 329 and 330:
338 Stirling of simple and improved
Page 331 and 332:
340 Stirling concentrations interfe
Page 333 and 334:
342 Daniels to sequence a 500-bp PC
Page 335 and 336:
344 Daniels 4. Load all of the samp
Page 337 and 338:
346 Daniels References 1. Orita, M.
Page 339 and 340:
348 Kösel et al. Fig. 1. Schematic
Page 341 and 342:
350 Kösel et al. 3. Methods 3.1. P
Page 343 and 344:
352 Kösel et al. Fig. 2. Sequencin
Page 345 and 346:
354 Kösel et al. 5. Kwok, S. and H
Page 347 and 348:
356 Mazars and Theillet Fig. 1. Sch
Page 349 and 350:
358 Mazars and Theillet of genomic
Page 351 and 352:
360 Mazars and Theillet
Page 353 and 354:
362 Suomalainen and Syvänen Fig. 1
Page 355 and 356:
364 Suomalainen and Syvänen detect
Page 357 and 358:
366 Suomalainen and Syvänen temper
Page 359 and 360:
368 Shen et al. ladder. Also, direc
Page 361 and 362:
370 Shen et al. 3. Mineral oil. 4.
Page 363 and 364:
372 Shen et al. extended primers, w
Page 365 and 366:
374 Quivy and Becker Fig. 1. The us
Page 367 and 368:
376 Quivy and Becker that decreases
Page 369 and 370:
378 Quivy and Becker 2. Solution of
Page 371 and 372:
380 Quivy and Becker 7. Precipitate
Page 373 and 374:
382 Quivy and Becker 7. Prepare a p
Page 375 and 376:
384 Quivy and Becker
Page 377 and 378:
386 Walsh by most, but not all M13
Page 379 and 380:
388 Walsh PCR products are now flus
Page 381 and 382:
390 Walsh 5. For palindromic restri
Page 383 and 384:
392 Walsh
Page 385 and 386:
394 McAleer, Coffey, and Dunham Fig
Page 387 and 388:
396 McAleer, Coffey, and Dunham Tab
Page 389 and 390:
398 McAleer, Coffey, and Dunham Tab
Page 391 and 392:
400 McAleer, Coffey, and Dunham
Page 393 and 394:
402 Stirling 5. Homopolymer Regions
Page 395 and 396:
406 Speel, Ramaekers, and Hopman Ta
Page 397 and 398:
408 Speel, Ramaekers, and Hopman Fi
Page 399 and 400:
410 Speel, Ramaekers, and Hopman po
Page 401 and 402:
Table 2 Comparison of PRINS, in Sit
Page 403 and 404:
414 Speel, Ramaekers, and Hopman te
Page 405 and 406:
Table 3 Summary of Control Experime
Page 407 and 408:
418 Speel, Ramaekers, and Hopman 3.
Page 409 and 410:
420 Speel, Ramaekers, and Hopman ad
Page 411 and 412:
422 Speel, Ramaekers, and Hopman 25
Page 413 and 414:
Page 415 and 416:
426 Bull and Paskins Fig. 1. Result
Page 417 and 418:
428 Bull and Paskins 2.3. Detection
Page 419 and 420:
430 Bull and Paskins 6. Shake the s
Page 421 and 422:
432 Bull and Paskins
Page 423 and 424:
434 Wiedorn and Goldmann Fig. 1. IS
Page 425 and 426:
436 Wiedorn and Goldmann 41. Protei
Page 427 and 428:
438 Wiedorn and Goldmann 2. Incubat
Page 429 and 430:
440 Wiedorn and Goldmann 3.15. Prim
Page 431 and 432:
442 Wiedorn and Goldmann ficient se
Page 433 and 434:
444 Wiedorn and Goldmann 25. Kommin
Page 435 and 436:
446 Gilchrist and Befus Fig. 1. Sch
Page 437 and 438:
448 Gilchrist and Befus 2. Cells ar
Page 439 and 440:
450 Gilchrist and Befus Fig. 3. RT
Page 441 and 442:
452 Gilchrist and Befus References
Page 443 and 444:
454 Speel, Ramaekers, and Hopman of
Page 445 and 446:
456 Speel, Ramaekers, and Hopman Fi
Page 447 and 448:
Page 449 and 450:
Page 451 and 452:
462 Speel, Ramaekers, and Hopman bu
Page 453 and 454:
Page 455 and 456:
468 Pearson and Stirling into PCR p
Page 457 and 458:
470 Wang 2. Materials 2.1. PCR 1. D
Page 459 and 460:
472 Wang Fig. 1. A brief outline of
Page 461 and 462:
474 Wang
Page 463 and 464:
476 Horton, Raju, and Conti-Fine Fi
Page 465 and 466:
478 Horton, Raju, and Conti-Fine th
Page 467 and 468:
480 Horton, Raju, and Conti-Fine 1.
Page 469 and 470:
482 Horton, Raju, and Conti-Fine ot
Page 471 and 472:
484 Horton, Raju, and Conti-Fine
Page 473 and 474:
486 Preston amplification approach
Page 475 and 476:
488 Preston 1. 10× PCR buffer: 100
Page 477 and 478:
490 Preston Fig. 1. Design of degen
Page 479 and 480:
492 Preston 2. Remove the AmpliTaq
Page 481 and 482:
494 Preston 3.3. Cloning and DNA Se
Page 483 and 484:
496 Preston MgCl 2 concentrations b
Page 485 and 486:
498 Preston 21. Hung, T., Mak, K.,
Page 487 and 488:
500 Ravassard et al. Fig. 1. Constr
Page 489 and 490:
502 Ravassard et al. size dispersio
Page 491 and 492:
504 Ravassard et al. 9. ddH 2 O. 10
Page 493 and 494:
506 Ravassard et al. 3.2.2. RNA Mix
Page 495 and 496:
508 Ravassard et al. 4. Wash twice
Page 497 and 498:
510 Ravassard et al.
Page 499 and 500:
512 Pont-Kingdon Fig. 1. Constructi
Page 501 and 502:
514 Pont-Kingdon 9. Invert tube and
Page 503 and 504:
516 Pont-Kingdon
Page 505 and 506:
518 Jones and Winistorfer Fig. 1. D
Page 507 and 508:
520 Jones and Winistorfer Fig. 2. D
Page 509 and 510:
522 Jones and Winistorfer The yield
Page 511 and 512:
524 Jones and Winistorfer 7. Goulde
Page 513 and 514:
526 Burke and Barik Fig. 1. The bas
Page 515 and 516:
528 Burke and Barik concentration o
Page 517 and 518:
530 Burke and Barik purified mutant
Page 519:
532 Burke and Barik
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