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OM t of c.iii - Vision Research Coordinating Center - Washington ...

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2/22/96 Chapter 10 Fluorescein Photodocumentation page 10-1<br />

10.1 Introduction<br />

Chapter 10<br />

Photodocumentation <strong>of</strong> Fluorescein Pattern<br />

Fluorescein photography allows for objective verification <strong>of</strong> the habitual rigid<br />

contact lens fit for rigid contact lens wearing patients enrolled in the CLEK Study. At<br />

least one person at each Participating Clinic is trained and certified to perform<br />

fluorescein photography. Fluorescein photography is performed at all Study Visits and<br />

when directed by the CLEK Photography Reading <strong>Center</strong> or the <strong>Coordinating</strong> <strong>Center</strong><br />

for rephotography as per the CLEK Photography Reading <strong>Center</strong> Operations Manual.<br />

10.2 Fluorescein Photography Technique<br />

The CLEK Photography Reading <strong>Center</strong> Coordinator provides, as needed, rolls<br />

<strong>of</strong> Kodak Ektachrome 100 ASA film to each CLEK Participating Clinic. It is advised that<br />

the film be placed in a cool, dry location.<br />

The Clinic Photographer loads the film into the camera, and the Photographer<br />

turns on the Nikon FS-2 slit lamp and the Nikon FS-2 flash power source (or other<br />

photographic system used for certification).<br />

The Clinic Photographer adheres to the following fluorescein photography<br />

protocol:<br />

(1) Clean the slit lamp biomicroscope chin and head rests.<br />

(2) Verify that the flash intensity is set at 5.<br />

(3) Verify that the beam splitter is in.<br />

(4) Verify that the magnification is set at 16X.<br />

(5) Verify the background illuminator is <strong>of</strong>f.<br />

(6) Verify that the aperture is wide open.<br />

(7) Verify that the cobalt filter is in place.<br />

(8) Place the Wratten 12 or Tiffen 2 yellow filter in front <strong>of</strong> the slit lamp’s<br />

objective lenses.<br />

(9) Assure the patient’s comfort and position in the slit lamp camera.

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