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6. DO ANY SPECIES BOUNDARIES...
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The vast of different DNA markers enabled to construct the linkage map with the quite
uniform distribution of markers and good coverage of the whole map distance. The only one
gap of 10 cM was observed and none big cluster of markers was found. Although several
small clusters of 3-4 markers could be observed the gaps between them were successfully
filled by the other types of markers. A nice example included two small clusters of AFLP
markers located between 45.7 cM and 60.9 cM on LG2 (Figure 6.5B), however a gap between
them was filled by the RAPD marker, OPB20-6, and ISJ marker ISJ4-7.
Surprisingly, RAPD and ISJ markers showed the most uniform distribution between all
linkage groups (Table 6.7). It is worthy to note that some clustering between AFLP and SSAP
markers was expected because the former markers are mixture of SSR, transposon and
restriction site markers. It was therefore, somehow unexpected that AFLPs tended to group
rather with RAPDs than transposon-based markers. On the other hand transposon-based
markers were not equally distributed between linkage groups. They were more frequent on
LG2, LG4 and LG5 than on the other linkage groups. Markers specific to Tpo1, DNA transposon
from the CACTA family, were clustered on the distance of 4 cM at the end of LG5. It
should be also realized that katG markers revealed by primers complementary to the bacterial
catalase-peroxidase gene were predominantly observed in close proximity to enzymatic
loci.
6.3.3. Segregation distortions in L. multiflorum and L. perenne crosses
Segregation distortions in the interspecific mapping population, BR3 x NZ15
Genotyping of 269 F 2
individuals from the cross between two botanical taxa L. multiflorum
and L. perenne did not reveal substantial non-Mendelian inheritance across the majority
of DNA and enzymatic loci. Single-locus segregations were consisted with the expected 3:1