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298 13. SUPPLEMENTARY MATERIALS ANNEX 13.13. BACTERIA SPECIFIC AMPLIFICATION POLYMORPHISM (B-SAP) The system is based on the assumption that some plant genes including those encoding peroxidases of the class I have been derived from a common prokaryotic ancestor and they likely evolved through multiple duplications, conversions and translocations. The utility of primers derived from KatG gene encoding catalase- peroxidase and insertional element IS6110 of M. tuberculosis has been confirmed in phylogenetic studies of Poaceae, closely related Pinus taxa, liverworts from the genus Pellia and several others (Zielinski and Polok 2005). In the present work four types of bacteria-derived primers were used: • 12 pairs of primers complementary to KatG gene of M. tuberculosis; • a pair of outer primers complementary to IS6110 of M. tuberculosis; • a pair of primers complementary to rpo gene of of M. tuberculosis; • a pair of primers complementary to pol gene of of M. tuberculosis. The PCR conditions were optimised in earlier studies (Zielinski and Polok 2005; Krzakowa et al. 2007). Each PCR reaction was performed in a total volume 20 µl. Concentrations of reagents and common thermal conditions are shown in Table 13.8.1. Number of cycles, annealing temperature and the time of elongation depended on pair of primers used. PCR products were loaded on 1.5% (w/v) agarose gels containing 0.5 µg/ml ethidium bromide, separated in 1 x TBE buffer (Tris-Borate-EDTA; Sambrook et al. 1989) at 100 V constant power, visualized under UV light (312 nm), photographed and stored as .jpg files.
13. SUPPLEMENTARY MATERIALS 299 All bands that could be reliable read were scored either present (1) or absent (0). The polymorphic products were referred as follow: • - katG1-2 when referring to a band revealed by a set of katG primers n o -1 and the second product from anode • - IS-1 when referring to a product number revealed by IS6110 primers counting from anode • - rpo1 when referring to a product number revealed by rpo primers counting from anode • - pol1 when referring to a product number revealed by pol primers counting from anode The dominant mode of inheritance of markers generated by katG and IS6110 primers used in this study was verified in F 1 and F 2 samples derived from four intra- and interspecific Lolium crosses. The presence of a band was dominant over the lack of it.
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This research was done within the E
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Acknowledgements I would like to th
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8 CONTENTS 4.4.4. Role of transposo
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10 CONTENTS 9.3.2. Similarity of ge
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12 1. INTRODUCTION
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14 1. INTRODUCTION occasionally use
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16 1. INTRODUCTION On the basis of
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18 1. INTRODUCTION The evidences fr
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20 1. INTRODUCTION overlap between
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22 1. INTRODUCTION et al. 2000; Cat
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24 1. INTRODUCTION According to the
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26 1. INTRODUCTION 1.6. APPLICATION
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28 1. INTRODUCTION of evolution wit
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30 1. INTRODUCTION are continuously
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2. GOALS The aim of this research w
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3. MORPHOLOGICAL VARIATION OF L. MU
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36 3. MORPHOLOGICAL VARIATION... fo
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38 3. MORPHOLOGICAL VARIATION...
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40 3. MORPHOLOGICAL VARIATION... Wi
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42 3. MORPHOLOGICAL VARIATION... va
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44 3. MORPHOLOGICAL VARIATION... 3.
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46 3. MORPHOLOGICAL VARIATION...
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48 3. MORPHOLOGICAL VARIATION... in
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50 3. MORPHOLOGICAL VARIATION... pl
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52 3. MORPHOLOGICAL VARIATION... di
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4. GENETIC DIVERSITY OF L. MULTIFLO
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56 4. GENETIC DIVERSITY... deed con
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58 4. GENETIC DIVERSITY... 4.2.2. D
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60 4. GENETIC DIVERSITY...
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64 4. GENETIC DIVERSITY... RAPD and
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66 4. GENETIC DIVERSITY... SSAP pol
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68 4. GENETIC DIVERSITY... It can b
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72 4. GENETIC DIVERSITY... than wit
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82 4. GENETIC DIVERSITY... power of
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84 4. GENETIC DIVERSITY... Hamrick
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86 4. GENETIC DIVERSITY... deviatio
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88 4. GENETIC DIVERSITY... individu
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90 4. GENETIC DIVERSITY... (inversi
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92 4. GENETIC DIVERSITY... related
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94 4. GENETIC DIVERSITY... cies and
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96 4. GENETIC DIVERSITY... their sp
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98 4. GENETIC DIVERSITY... 4.5. CON
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100 5. ORIGIN OF SEEDLING... Applic
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102 5. ORIGIN OF SEEDLING... 5.2.2.
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104 5. ORIGIN OF SEEDLING... 5.3. R
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106 5. ORIGIN OF SEEDLING... very l
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108 5. ORIGIN OF SEEDLING... 5.3.4.
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110 5. ORIGIN OF SEEDLING... 5.4. D
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112 5. ORIGIN OF SEEDLING... A seco
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114 5. ORIGIN OF SEEDLING... Perhap
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6. DO ANY SPECIES BOUNDARIES EXIST
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118 6. DO ANY SPECIES BOUNDARIES...
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7. ROLE OF QTL s IN THE EARLY EVOLU
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160 7. ROLE OF QTLs IN THE EARLY EV
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196 8. MOLECULAR PHYLOGENY OF THE G
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9. PHYLOGENETIC RELATIONSHIPS BETWE
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224 9. PHYLOGENETIC RELATIONSHIPS..
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10. MARKER EFFICIENCY 10.1. INTRODU
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238 10. MARKER EFFICIENCY 10.3. DNA
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240 10. MARKER EFFICIENCY method. H
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242 10. MARKER EFFICIENCY alleles p
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244 10. MARKER EFFICIENCY proportio
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246 10. MARKER EFFICIENCY 10.7. CON
Page 248 and 249: 248 11. CONCLUSIONS ABOUT EVOLUTION
Page 250 and 251: 250 12, LITERATURE CITED Bączkiewi
Page 252 and 253: 252 12, LITERATURE CITED Clayton WD
Page 254 and 255: 254 12, LITERATURE CITED Giddings G
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Page 258 and 259: 258 12, LITERATURE CITED MacDonald
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Page 262 and 263: 262 12, LITERATURE CITED Schiex T,
Page 264 and 265: 264 12, LITERATURE CITED United Sta
Page 266 and 267: 266 12, LITERATURE CITED Zielinski
Page 268 and 269: 268 13. SUPPLEMENTARY MATERIALS pla
Page 270 and 271: 270 13. SUPPLEMENTARY MATERIALS
Page 274 and 275: 274 13. SUPPLEMENTARY MATERIALS ANN
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Page 290 and 291: 290 13. SUPPLEMENTARY MATERIALS •
Page 292 and 293: 292 13. SUPPLEMENTARY MATERIALS The
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Page 304 and 305: The average gene diversity between
Page 306 and 307: 306 13. SUPPLEMENTARY MATERIALS Unb
Page 308 and 309: 308 14. ABBREVIATIONS Lolcopia1 - L
Page 310 and 311: 310 14. ABBREVIATIONS Drosophila wi
Page 312 and 313: 312 14. ABBREVIATIONS Saccharomyces
Page 314 and 315: 314 15. SUMMARY At different stages
Page 316 and 317: 316 15. SUMMARY L. multiflorum mito
Page 318 and 319: REVIEWERS’ COMMENTS With a great
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