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238 10. MARKER EFFICIENCY 10.3. DNA PURITY AND MARKER REPRODUCIBILTY The high purity of DNA is sine qua non to obtain reproducible results for any marker category. The common view is that some methods perceived as less sophisticated (RAPD or similar) do not need high purity of DNA and consequently many rapid protocols and commercial kits are used. On the other hand RAPDs have developed a reputation of poor reproducibility. As a result, having bad own experience, many reviewers reject papers based on RAPDs as unreliable. Conversely, own experience was that 100% of reproducibility can be obtained provided DNA is of high quality. Every time the isolation procedure was simplified, the results were much worse. Generally, commercial kits are not suitable for isolation. Instead
10. MARKER EFFICIENCY 239 the CTAB method with additional cleaning by cesium chloride should be used. For more troublesome species including liverworts, pines, roses the addition of PVP is recommended to reduce the level of phenols that have tremendous effect on RAPD quality. Note, that getting the procedure longer than in the original protocol e.g., precipitation time lengthening, precipitation with ethanol instead of isopropanol improves considerably the DNA purity and quality. Once DNA is isolated it can be used for many purposes, and the RAPD can be the simplest
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This research was done within the E
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Acknowledgements I would like to th
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8 CONTENTS 4.4.4. Role of transposo
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10 CONTENTS 9.3.2. Similarity of ge
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12 1. INTRODUCTION
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14 1. INTRODUCTION occasionally use
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16 1. INTRODUCTION On the basis of
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18 1. INTRODUCTION The evidences fr
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20 1. INTRODUCTION overlap between
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22 1. INTRODUCTION et al. 2000; Cat
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24 1. INTRODUCTION According to the
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26 1. INTRODUCTION 1.6. APPLICATION
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28 1. INTRODUCTION of evolution wit
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30 1. INTRODUCTION are continuously
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2. GOALS The aim of this research w
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3. MORPHOLOGICAL VARIATION OF L. MU
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36 3. MORPHOLOGICAL VARIATION... fo
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38 3. MORPHOLOGICAL VARIATION...
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40 3. MORPHOLOGICAL VARIATION... Wi
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42 3. MORPHOLOGICAL VARIATION... va
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44 3. MORPHOLOGICAL VARIATION... 3.
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46 3. MORPHOLOGICAL VARIATION...
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48 3. MORPHOLOGICAL VARIATION... in
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50 3. MORPHOLOGICAL VARIATION... pl
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52 3. MORPHOLOGICAL VARIATION... di
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4. GENETIC DIVERSITY OF L. MULTIFLO
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56 4. GENETIC DIVERSITY... deed con
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58 4. GENETIC DIVERSITY... 4.2.2. D
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60 4. GENETIC DIVERSITY...
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64 4. GENETIC DIVERSITY... RAPD and
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66 4. GENETIC DIVERSITY... SSAP pol
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68 4. GENETIC DIVERSITY... It can b
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72 4. GENETIC DIVERSITY... than wit
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82 4. GENETIC DIVERSITY... power of
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84 4. GENETIC DIVERSITY... Hamrick
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86 4. GENETIC DIVERSITY... deviatio
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88 4. GENETIC DIVERSITY... individu
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90 4. GENETIC DIVERSITY... (inversi
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92 4. GENETIC DIVERSITY... related
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94 4. GENETIC DIVERSITY... cies and
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96 4. GENETIC DIVERSITY... their sp
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98 4. GENETIC DIVERSITY... 4.5. CON
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100 5. ORIGIN OF SEEDLING... Applic
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102 5. ORIGIN OF SEEDLING... 5.2.2.
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104 5. ORIGIN OF SEEDLING... 5.3. R
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106 5. ORIGIN OF SEEDLING... very l
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108 5. ORIGIN OF SEEDLING... 5.3.4.
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110 5. ORIGIN OF SEEDLING... 5.4. D
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112 5. ORIGIN OF SEEDLING... A seco
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114 5. ORIGIN OF SEEDLING... Perhap
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6. DO ANY SPECIES BOUNDARIES EXIST
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118 6. DO ANY SPECIES BOUNDARIES...
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7. ROLE OF QTL s IN THE EARLY EVOLU
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160 7. ROLE OF QTLs IN THE EARLY EV
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Page 188 and 189: 188 7. ROLE OF QTLs IN THE EARLY EV
Page 196 and 197: 196 8. MOLECULAR PHYLOGENY OF THE G
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Page 224 and 225: 224 9. PHYLOGENETIC RELATIONSHIPS..
Page 236 and 237: 10. MARKER EFFICIENCY 10.1. INTRODU
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Page 248 and 249: 248 11. CONCLUSIONS ABOUT EVOLUTION
Page 250 and 251: 250 12, LITERATURE CITED Bączkiewi
Page 252 and 253: 252 12, LITERATURE CITED Clayton WD
Page 254 and 255: 254 12, LITERATURE CITED Giddings G
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Page 258 and 259: 258 12, LITERATURE CITED MacDonald
Page 260 and 261: 260 12, LITERATURE CITED Payne RC,
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Page 266 and 267: 266 12, LITERATURE CITED Zielinski
Page 268 and 269: 268 13. SUPPLEMENTARY MATERIALS pla
Page 270 and 271: 270 13. SUPPLEMENTARY MATERIALS
Page 274 and 275: 274 13. SUPPLEMENTARY MATERIALS ANN
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288 13. SUPPLEMENTARY MATERIALS ANN
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290 13. SUPPLEMENTARY MATERIALS •
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292 13. SUPPLEMENTARY MATERIALS The
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294 13. SUPPLEMENTARY MATERIALS
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296 13. SUPPLEMENTARY MATERIALS fie
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302 13. SUPPLEMENTARY MATERIALS Ann
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The average gene diversity between
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306 13. SUPPLEMENTARY MATERIALS Unb
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308 14. ABBREVIATIONS Lolcopia1 - L
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310 14. ABBREVIATIONS Drosophila wi
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312 14. ABBREVIATIONS Saccharomyces
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314 15. SUMMARY At different stages
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316 15. SUMMARY L. multiflorum mito
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REVIEWERS’ COMMENTS With a great
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