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8. MOLECULAR PHYLOGENY OF THE GENUS LOLIUM
lotypes could not be treated as species specific. They should rather be perceived as the typical
feature of allogamous species. In spite of diverse haplotypes a certain degree of similarity
could be found. The haplotypes of L. perenne ssp. perenne and L. rigidum were mostly the
same with the only one additional band in perenne. Another subspecies, multiflorum apart
from a common pattern typical of perenne and L. rigidum had additional five bands. All these
bands were shared with L. loliaceum and moreover three with P. pratensis. Closely related
with Lolium, F. pratensis had all bands but one typical of Lolium.
To study in more details relationships between Lolium species a mitochondrial gene
derived from L. perenne ssp. multiflorum was amplified and after confirming that a single
product was obtained, it was subjected to restriction analyses. Among seven Lolium
taxa studied, only four gave a single band. These were all allogamous species (multiflorum,
perenne and L. rigidum) in addition to autogamous L. loliaceum. Moreover, a single