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15. SUMMARY
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A by-product of all these analyses was the elaboration of a new marker system based
on different bacterial genes, but mainly on catalase-peroxidase from M. tuberculosis. The
system proved to be highly effective in phylogenetic analyses and revealing species specific
markers. The resolution of the tree based on katG markers was comparable to that
based on multi-marker approach and therefore the system can be used an alternative to
using many different marker types. Genetic mapping studies enabled to locate markers
derived from bacteria on Lolium linkage map. The markers had the dominant mode of
inheritance i.e., the presence of a band is dominant over its lack. Moreover, these types
of markers rarely show segregation distortions. The linkage between katG markers and
peroxidase loci support the idea that katG primers complementary to the M. tuberculosis
catalase-peroxidase gene amplify plant peroxidases. The linkage between katG and the
other enzymatic loci proves that these markers are predominantly correlated with genes
encoding enzymes. It was proposed to name this method as Bacteria Specific Amplification
Polymorphism (B-SAP). And finally, all these analysis were coupled with estimation
of marker efficiency with respect to the costs and utility in different areas of genetic evolutionary
research.