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4. GENETIC DIVERSITY...
SSAP polymorphism
The primer complementary to L. perenne CACTA transposon, Tpo1 produced a great
number of bands in L. multiflorum and L. perenne thus, confirming the high abundance of
Tpo1 in these two ryegrasses. Similarly, each of the two LTR primers complementary to Ty1-
copia consensus sequences produced bands on SSAP gel for each species, showing that
each transposon is present within them (Figure 4.4A). Therefore, the transposons revealed
by LTR1 and LTR2 were further assigned as Lolcopia1 and Lolcopia2, respectively.
All transposon sequences studied were highly polymorphic among L. multiflorum and
L. perenne (Table 4.1), indicating that they have been transposing within these species in
the recent past. The overall polymorphism level for each transposon was the same for Tpo1
and Lolcopia1 i.e., 93% and 88% for Lolcopia2. The proportion of polymorphic sites and the