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200 8. MOLECULAR PHYLOGENY OF THE GENUS LOLIUM lotypes could not be treated as species specific. They should rather be perceived as the typical feature of allogamous species. In spite of diverse haplotypes a certain degree of similarity could be found. The haplotypes of L. perenne ssp. perenne and L. rigidum were mostly the same with the only one additional band in perenne. Another subspecies, multiflorum apart from a common pattern typical of perenne and L. rigidum had additional five bands. All these bands were shared with L. loliaceum and moreover three with P. pratensis. Closely related with Lolium, F. pratensis had all bands but one typical of Lolium. To study in more details relationships between Lolium species a mitochondrial gene derived from L. perenne ssp. multiflorum was amplified and after confirming that a single product was obtained, it was subjected to restriction analyses. Among seven Lolium taxa studied, only four gave a single band. These were all allogamous species (multiflorum, perenne and L. rigidum) in addition to autogamous L. loliaceum. Moreover, a single
8. MOLECULAR PHYLOGENY OF THE GENUS LOLIUM 201 band was observed in F. pratensis. The rest of studied species gave a multi-band pattern that confirmed their distinctiveness. Restriction digestions were carried out only for species that amplified a single band. Restriction maps consisted from sites specific to five enzymes, AluI, HinfI, HindIII, MspI and TaqI. The number of sites ranged from 13 in L. loliaceum to 17 in F. pratensis. The majority of sites were common for all or for the most of species (Figure 8.3). F. pratensis had only one unique TaqI site i.e., not observed in
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This research was done within the E
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Acknowledgements I would like to th
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8 CONTENTS 4.4.4. Role of transposo
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10 CONTENTS 9.3.2. Similarity of ge
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12 1. INTRODUCTION
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14 1. INTRODUCTION occasionally use
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16 1. INTRODUCTION On the basis of
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18 1. INTRODUCTION The evidences fr
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20 1. INTRODUCTION overlap between
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22 1. INTRODUCTION et al. 2000; Cat
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24 1. INTRODUCTION According to the
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26 1. INTRODUCTION 1.6. APPLICATION
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28 1. INTRODUCTION of evolution wit
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30 1. INTRODUCTION are continuously
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2. GOALS The aim of this research w
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3. MORPHOLOGICAL VARIATION OF L. MU
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36 3. MORPHOLOGICAL VARIATION... fo
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38 3. MORPHOLOGICAL VARIATION...
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40 3. MORPHOLOGICAL VARIATION... Wi
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42 3. MORPHOLOGICAL VARIATION... va
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44 3. MORPHOLOGICAL VARIATION... 3.
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46 3. MORPHOLOGICAL VARIATION...
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48 3. MORPHOLOGICAL VARIATION... in
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50 3. MORPHOLOGICAL VARIATION... pl
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52 3. MORPHOLOGICAL VARIATION... di
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4. GENETIC DIVERSITY OF L. MULTIFLO
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56 4. GENETIC DIVERSITY... deed con
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58 4. GENETIC DIVERSITY... 4.2.2. D
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60 4. GENETIC DIVERSITY...
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64 4. GENETIC DIVERSITY... RAPD and
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66 4. GENETIC DIVERSITY... SSAP pol
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68 4. GENETIC DIVERSITY... It can b
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72 4. GENETIC DIVERSITY... than wit
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82 4. GENETIC DIVERSITY... power of
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84 4. GENETIC DIVERSITY... Hamrick
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86 4. GENETIC DIVERSITY... deviatio
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88 4. GENETIC DIVERSITY... individu
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90 4. GENETIC DIVERSITY... (inversi
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92 4. GENETIC DIVERSITY... related
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94 4. GENETIC DIVERSITY... cies and
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96 4. GENETIC DIVERSITY... their sp
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98 4. GENETIC DIVERSITY... 4.5. CON
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100 5. ORIGIN OF SEEDLING... Applic
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102 5. ORIGIN OF SEEDLING... 5.2.2.
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104 5. ORIGIN OF SEEDLING... 5.3. R
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106 5. ORIGIN OF SEEDLING... very l
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108 5. ORIGIN OF SEEDLING... 5.3.4.
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110 5. ORIGIN OF SEEDLING... 5.4. D
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112 5. ORIGIN OF SEEDLING... A seco
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114 5. ORIGIN OF SEEDLING... Perhap
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6. DO ANY SPECIES BOUNDARIES EXIST
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118 6. DO ANY SPECIES BOUNDARIES...
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Page 150 and 151: 150 6. DO ANY SPECIES BOUNDARIES...
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Page 248 and 249: 248 11. CONCLUSIONS ABOUT EVOLUTION
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250 12, LITERATURE CITED Bączkiewi
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252 12, LITERATURE CITED Clayton WD
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254 12, LITERATURE CITED Giddings G
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256 12, LITERATURE CITED Jing R, Kn
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258 12, LITERATURE CITED MacDonald
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260 12, LITERATURE CITED Payne RC,
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262 12, LITERATURE CITED Schiex T,
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264 12, LITERATURE CITED United Sta
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266 12, LITERATURE CITED Zielinski
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268 13. SUPPLEMENTARY MATERIALS pla
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270 13. SUPPLEMENTARY MATERIALS
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274 13. SUPPLEMENTARY MATERIALS ANN
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280 13. SUPPLEMENTARY MATERIALS Ann
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290 13. SUPPLEMENTARY MATERIALS •
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292 13. SUPPLEMENTARY MATERIALS The
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296 13. SUPPLEMENTARY MATERIALS fie
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The average gene diversity between
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306 13. SUPPLEMENTARY MATERIALS Unb
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308 14. ABBREVIATIONS Lolcopia1 - L
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310 14. ABBREVIATIONS Drosophila wi
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312 14. ABBREVIATIONS Saccharomyces
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314 15. SUMMARY At different stages
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316 15. SUMMARY L. multiflorum mito
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REVIEWERS’ COMMENTS With a great
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