Untitled

8. MOLECULAR PHYLOGENY OF THE GENUS LOLIUM
203
tion with HaeIII gave quite surprising results. Two self-pollinating species, L. remotum and
L. temulentum had identical patterns but clearly distinct from outbreeders (Figure 8.4). The
unexpected findings involved the identity of their patterns with that of F. pratensis. Another
point was that another self-pollinating species L. loliaceum had the pattern typical to outpollinating
taxa, multiflorum, perenne and L. rigidum. In summary, the ITS regions enabled
to divide genus into auto- and allogamous species. In addition, L. persicum was clearly different
from L. remotum and L. temulentum. Further distinction between these two species
as well as between outbreeders was not possible. Moreover, the position of self-pollinating
L. loliaceum was in disagreement with taxonomic position owing to high similarity to allogamous
species.
The placement of L. loliaceum together with out-pollinated taxa was confirmed by the
analysis of intergenic spacer between genes encoding leucine tRNA (Figure 8.5). With this
only exception, the t-RNA based tree grouped auto- and allogamous species quite well.
Unsurprisingly, two subspecies of L. perenne were grouped together and then formed
a cluster with L. loliaceum, that further joined with F. pratensis. The other cluster consisted
from autogamous species with L. remotum and L. temulentum joining at the highest similarities
and more distant L. persicum. As expected P. pratensis occupied the most external
position. Strangely, but L. rigidum adopted slightly distant position from the rest Loli-