Seed Health Management for Better Productivity - Govind Ballabh ...

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(Seed Health Management for Better Productivity)‣ Production of hybrid seed.‣ Maintenance of inbred parents.Methods of Hybrid Seed Production in Cucurbits1. Hand pollination – a. Without emasculationb. With emasculation2. Defloration and insect pollination3. Use of gynoecious lines and insect pollination4. Use of genetic male sterility – utilization of morphological markers5. Use of monoecious line6. Chemical suppression of male flowers and open pollination.1. Hand Pollination: a. Without EmasculationApplicable for monoecious cucurbits (Cucumber, Squash, Pumpkin, Bitter gourd)Hand Pollination: b. With EmasculationApplicable for andromonoecious cucurbits1942- Munger – Hybrid seed production in melonsFemale plantHermaphrodite flowerEmasculation + baggingCover with cottonMale plantMale flower(Before anthesis)Pollination (after anthesis)Planting ratio: 6:1Bagging – F 1 seed collected2. Defloration and Insect PollinationCucumber, Watermelon, Pumpkin and Gourds (Bottle gourd, Ash & Bitter gourd)♀Seed parent♂Pollen parentRemoval of ♂ flowerbefore anthesis♂ flowerNatural cross pollination by beeF1 SeedsOne medium sized bee colony/ha is enough for good HSPIsolation distance: 400mPlanting ratio:Bottle gourd: 3:1Squash: 4:1- 88 -
(Seed Health Management for Better Productivity)3. Use of Gynoecious Lines and Insect Pollination Planting ratio (3 gynoecious female line: 1 pollinator line). Natural pollination by bees. Any other variety except parents should not be there. Blending – to improve pollination in gynoecious hybrid seeds (10% monoecious types. Sumter cultivar – most common blender. If parthenocarpic gynoecious hybrid – no blending.Commercial gynoecious hybrids In cucumber Pusa Sanyog, DCH-1, 2 (T.A. More and V.S. Sheshadri in early ninties), Phule Prachi (Gyc-2) and Phule Champa (Gyc-4) (More, 2002) In muskmelon MH-10 (Dhatt et al.,2005)4. Use of Genetic Male Sterility• Planting ratio (2 female : 1 male)• Planting of 6 seedlings per hill for seed parent• Pierce anthesised flowers from female parent• Tag male sterile plants• Rouging of male plants for 8-10 days• Allow natural pollination• Punjab Hybrid-1 – ms -1 x Hara MadhuMaintenance: Rouging of male fertile plants from female line by morphological marker trait (malesterility associated with glabrous foliage in watermelon)5. Chemical Suppression of Male Flowers Planting ratio : 5:1 in Cucurbita pepo 2-true leaf stage is most responsive for application of chemicals Natural pollination/hand pollination.6. Foliar Spray of PGRS to Induce Increased Proportion of Pistillate FlowersPGRConc (mg/l) CucurbitsCycocel (CCC) 250-500 Most cucurbits, effective in cucumberEthephon (CEPA) 150-200 Most cucurbitsGibberellic Acid (GA) 150-200 WatermelonIndole acetic acid (IAA) 10 Snake gourd & bitter gourdNAA 20-200 Cucumber, melons & gourdsMaleic hydrazide (MH) 25-10050-150Cucumber, muskmelon, bottle gourd, ridgegourd- 89 -
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potential. An improvement in qualit
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9. Session Arrangement CommitteeDr.
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9. Dr. Pradip Kumar BorahSr. Scient
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TRAININGONANNEXURE-IIISEED HEALTH M
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ANNEXURE-IVCENTRE OF ADVANCED STUDI
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SundayApril 6MondayApril 7TuesdayAp
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TuesdayApril 15WednesdayApril 16Thu
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