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METHOD ABSTRACTS203.1 / MULTI-RESIDUE MYCOTOXIN ANALYSISSINGLE RUN ANALYSIS OF DEOXYNIVALENOL, AFLATOXINS, OCHRATOXIN A,ZEARALENONE AND FUMONISIN BY HPLC AND POST-COLUMN DERIVATIZATIONMaria Otserova, PhD, Sareeta Nerkar, PhD, Michael Pickering, PhDAlthough Aspergillus (Aatoxins, Ochratoxin A) are generally associated with peanuts and Fusarium (Deoxynivalenol,Zearalenone) with wheat, these fungi and those that produce other toxins are not host selective and so can cross plant species.This situation is complicated by the fact that the microscopic mold may not be visible to the naked eye. Also, when infectedgrains are processed, any visible mold is lost but the toxic metabolites carry over into the nished products. Thus, multi-residueanalytical screens for toxins in grain and nished goods are a wiser choice than single-family protocols. We present a singlescreen to cover ve families of toxins. This method is suitable for analyzing beverages, grains and feeds.Sample Extraction and Clean-up25 g of nely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of ltered extractis diluted with 70 mL of Phosphate Buffered Saline (PBS). Aatoxins, Zearealenone and Ochratoxin A are isolated usingAOZ Immunoafnity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are elutedwith 2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoafnity column (Vicam, USA) according tothe procedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol.To isolate DON 3 mL of ltered extract is mixed with 6 mL of Acetonitrile and cleaned with MycoSep 277 column(Romer Labs, USA) according to the manufacture’s instructions. The cleaned solution is ltered and 5.5 mL of it is combinedwith eluants from AOZ and FumoniTest columns. The solution is evaporated to 0.5 mL and nal volume is adjustedto 1 mL with Methanol.METHODAnalytical ConditionsColumn: MYCOTOX reversed-phase C 18,4.6x250 mm Catalog No. 1612124Temperature: 40 ºCFlow Rate: 1 mL/minMobile Phase: Sodium Phosphate buffer, pH 3.5Catalog No 1700-1108/MeOH/ACNHPLC PROGRAMTIME (Min) 1700-1108 ,% METHANOL, % ACETONITRILE, %0.0 85 0 155.0 85 0 155.1 57 28 1520.0 57 28 1523.0 40 60 040.0 40 60 050.0 20 0 8060.0 20 0 80REFERENCES:Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thiex, N.,Multiresidue Mycotoxin Analysis in Corn Grain by ColumnHigh-performance Liquid Chromatography with Post-columnPhotochemical and Chemical Derivatization: Single-LaboratoryValidation., (2009), J AOAC Int., 92, 15-25Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 1.4 mLTemperature: 60 CReagent: OPA, Thiouor, Brij 35® in GA104Photochemical Reactor: UVEDetection:FluorescenceAatoxins (photochemical derivatization) ex= 365 nm; em= 455 nmFumonisins (post-column derivatization with OPA) ex= 330 nm; em= 465 nmOchratoxin A ex= 335 nm; em= 455 nmZearalenone ex= 275 nm; em= 455 nmUV/VisDeoxynivalenol=218 nm
METHOD ABSTRACTSFLD Signalreagent ONreagent OFFUV signalDONFB1reagent ONFB2G1G2 B2B1OchraZON0 10 20 30 40 50 minChromatogram of a standard solution of mycotoxins. Concentrations of toxins (ng/mL): deoxynivalenol (DON) 930,aatoxin B1 4.5, aatoxin B2 1.6, aatoxin G1 4.7, aatoxin G2 2, ochratoxin A 92, zearalenone 481, fumonisin B1474, and fumonisin B2 627.99FLD Signalreagent ONFB1reagent OFFUV signalreagent ONFB2DONG1G2 B2B1OchraZON0 10 20 30 40 50 minChromatogram of a corn grain sample naturally contaminated with fumonisins FB1 and FB2 and spiked with DON;aatoxins B1, B2, G1, and G2; ochratoxin A; and zearalenone. Concentrations of toxins in the sample (ng/g):deoxynivalenol 930, aatoxin B1 5.0, aatoxin B2 1.7, aatoxin G1 5.1, aatoxin G2 2.2, ochratoxin A 102,zearalenone 529, fumonisin B1 1838, and fumonisin B2 1107.PRODUCT CATALOGPICKERING LABORATORIES10/11
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TABLE OF CONTENTSINTRODUCTION3 Cust
Page 6 and 7:
INTRODUCTIONINSTALLATION & TRAINING
Page 8 and 9:
“A LOT HAS CHANGED SINCE PRODUCIN
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INSTRUMENTS & HARDWAREFEATURE HIGHL
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INSTRUMENTS & HARDWARE• Indoor us
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INSTRUMENTS & HARDWARECRX400 POST-C
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CHROMATOGRAMS, COLUMNS & GUARDSPick
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CHROMATOGRAMS, COLUMNS & GUARDSREVE
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CHROMATOGRAMS, COLUMNS & GUARDS70-M
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSSODI
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSCARB
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CHROMATOGRAMS, COLUMNS & GUARDSSEPA
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CHROMATOGRAMS, COLUMNS & GUARDSALKI
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CHROMATOGRAMS, COLUMNS & GUARDSCOND
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CHROMATOGRAMS, COLUMNS & GUARDSAFLA
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CHROMATOGRAMS, COLUMNS & GUARDSVOGL
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CHROMATOGRAMS, COLUMNS & GUARDSANAL
Page 50: “PURE CHEMICALS DO WHAT THEY ARE
Page 54 and 55: REAGENTS & CHEMICALSSERAPREP & URIP
Page 56 and 57: REAGENTS & CHEMICALSRESTORECHROMATO
Page 58 and 59: REAGENTS & CHEMICALSCALIBRATION STA
Page 60 and 61: ELUANTSCHROMATOGRAPHIC GRADEPickeri
Page 62 and 63: APPLICATION KITSAmino Acid Analysis
Page 64 and 65: BECKMAN 6300/7300 COLUMNS & CHEMICA
Page 66 and 67: REPLACEMENT COMPONENTS, FITTINGS &
Page 74 and 75: “THE LCTECH STORY”Pickering Lab
Page 76 and 77: LCTECH / GPC PRODUCT LINEGPC ULTRA
Page 78 and 79: LCTECH / GPC PRODUCT LINEUtilizing
Page 80 and 81: LCTECH / GPC PRODUCT LINE• Can be
Page 82 and 83: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 84 and 85: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 86 and 87: LCTECH / GPC PRODUCT LINE3Intensity
Page 88 and 89: LCTECH / GPC PRODUCT LINEEVA IIICON
Page 90 and 91: LCTECH / MYCOTOXIN PRODUCT LINEUV D
Page 92 and 93: LCTECH / MYCOTOXIN PRODUCT LINESAMP
Page 94 and 95: LCTECH / MYCOTOXIN PRODUCT LINEOTAR
Page 96 and 97: LCTECH / MYCOTOXIN PRODUCT LINEACCE
Page 98 and 99: “CUSTOMERS REALLY ONLY WANT THE C
Page 102 and 103: METHOD ABSTRACTS208 / MULTI-RESIDUE
Page 104 and 105: METHOD ABSTRACTS328 / ANALYSIS OF A
Page 106 and 107: METHOD ABSTRACTS382 / 70-MIN AMINO
Page 108 and 109: METHOD ABSTRACTS205 / AMINOGLYCOSID
Page 110 and 111: METHOD ABSTRACTS102 / ANALYSIS OF B
Page 112 and 113: METHOD ABSTRACTS120 / BROMATE ANALY
Page 114 and 115: METHOD ABSTRACTS108 / POST-COLUMN L
Page 116 and 117: METHOD ABSTRACTS112 / EXPANDED HPLC
Page 118 and 119: METHOD ABSTRACTS325 / ANALYSIS OF F
Page 120 and 121: METHOD ABSTRACTS206 continued from
Page 124 and 125: METHOD ABSTRACTS105.2 / PARALYTIC S
Page 126 and 127: METHOD ABSTRACTS104 / POLYETHER ANT
Page 128 and 129: METHOD ABSTRACTS124 / SULFONAMIDE A
Page 132 and 133: GENERAL TERMS & CONDITIONS1 CONTROL
Page 134: DISTRIBUTORSContinued from previous
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