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REAGENTS & CHEMICALSSERAPREP & URIPREPCHROMATOGRAPHIC GRADE REAGENTS FOR THE PREPARATIONOF NATIVE SAMPLES FOR AMINO ACID ANALYSISPreparation of samples such as serum, urine, other physiologicaluids, plant extracts, foods and beverages requires controlof pH and normality, and removal of soluble protein.The samples must be held <strong>to</strong> a narrow pH range between2.1 and 2.5, and at the proper Lithium ion concentration <strong>to</strong>ensure reproducibility in the early part of the chroma<strong>to</strong>gram.SERAPREP and URIPREP replace commonly usedprotein precipitation reagents such as Ace<strong>to</strong>nitrile, Perchloricacid, and Picric acid, and eliminate the need for dialysis,ultraltration, and the repeated centrifugation steps, followedby pH adjustment.SERAPREP is used for preparing serum and othersamples with high buffering capacity, e.g. sardine oil.URIPREP is used for urine and other samples with lowbuffering capacity, such as fruit juices, beer and wines. Theefciency of protein precipitation and need for post-centrifugationpH adjustment of the sample determine whichreagent is best for y<strong>our</strong> particular sample.SERAPREP & URIPREP CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYSP100 SERAPREP each (250 mL/bottle)UP100 URIPREP each (250 mL/bottle)HYDROLYSIS REAGENT CB130CHROMATOGRAPHIC GRADEThis 0.05 M NaOH/C47 reagent is applied in the rststage of post-column carbamate derivatization. At 100 ºCthe separated carbamates are converted from urethanes <strong>to</strong>Methylamine. The Methylamine can then react with OPAand Thiouor <strong>to</strong> form the characteristically uorescentisoindole.Hydrolysis Reagent contains C47 which prevents theprecipitation of insoluble minerals (e.g. Ca 2+ , Mg 2+ )from samples of hard drinking water and ground water.The C47 additive complexes these minerals and keepsthem in solution.HYDROLYSIS REAGENT CB130.2CHROMATOGRAPHIC GRADEThe new Hydrolysis Reagent CB130.2 is formulated according<strong>to</strong> USEPA Method 531.2 and contains 0.075 MSodium Hydroxide. This reagent is applied during the rststage of post-column derivatization for the analysis of carbamatepesticides.Hydrolysis Reagent CB130.2 contains C47, which preventsthe precipitation of insoluble minerals from samplesof hard drinking and ground water, and protects y<strong>our</strong>instruments from damage.HYDROLYSIS REAGENT CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYCB130 Hydrolysis Reagent for carbamateanalysis 0.05 Mcase of 4(950 mL/bottle)CB130.2Hydrolysis Reagent for carbamateanalysis 0.075 Mcase of 4(950 mL/bottle)

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