to download our North American Product Catalog - Pickering ...

CHROMATOGRAMS, COLUMNS & GUARDSALKION COLUMN ANALYSIS OF PARAQUAT ANDDIQUAT IN WATERPost-column Conditions:Reagent 1: 300 mg/L of Sodium Dithionite in0.3 M Sodium HydroxideReactor: 35 °C, 0.15 mLReagents Flow Rate: 0.3 mL/minDetection:UV-Vis Detector: 395 nm for PQ, 378 nm for DQCONDITIONSTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % i-PROPANOL %0 31.5 53.5 0 1510 28 47 10 1515 28 47 10 1515.1 0 85 10 517 0 85 10 517.1 31.5 53.5 0 1523 31.5 53.5 0 15FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 50 ºC,INJECTION VOLUME: up to several mLDQALKION COLUMN ANALYSIS OF STREPTOMYCINPost-column Conditions:Reagent 1: 0.75 N Sodium HydroxideReactor 1: ambient, 0.1 mLReagent 2: 0.6 % NinhydrinReactor 2: 60 °C, 0.5 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex395 nm, em500 nmorReagent 1: Oxidizing Reagent: 100 µL of 5 %Sodium Hypochlorite in GA116Reactor 1: 50 °C, 0.5 mLReagent 2: 300 mg of OPA, 2 g Thiouor, 3 mL of 30 %Brij® 35 solution in 950 mL of OD104Reactor 2: ambient, 0.1 mLDetection:Fluorometer: ex330 nm, em465 nmPQPQ=ParaquatDQ=DiquatCONDITIONSTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 010 60 40 010.1 48 48 415 48 48 415.1 60 40 021 60 40 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: up to several LStreptomycin68 10 12minNOTE: Above chromatogram is approximately 1 ppm in 200 µL.ALKION COLUMN CATALOG INFORMATIONCATALOG NO.DESCRIPTION9410917 ALKION Cation-exchange column, K form, 4.0 × 150 mm9493020 ALKION Guard column, K form, 3.0 × 20 mm0 5 10 15min