to download our North American Product Catalog - Pickering ...

METHOD ABSTRACTS380 / 30-MINUTE AMINO ACID ANALYSIS OF HYDROLYZED SAMPLESPickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Noother technique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrindetection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio foreach amino acid is a constant and so offers information about peak purity.Pinnacle PCX post-column derivatization system provides a unique opportunity to combine eluant gradient capabilitiesof modern HPLC instruments with column temperature gradients. As might be expected, this capability also reduces theanalysis time.We introduce an accelerated Amino Acid analysis method for hydrolyzed samples that utilizes temperature andeluant gradients.METHODAnalytical ConditionsColumn: High-efciency Sodiumcation-exchange column, 4.6 x 110 mm,Catalog number 1154110TFlow Rate: 0.6 mL/minMobile Phase: Na315, Na740, RG011Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.5 mLCOLUMN OVEN PROGRAMTIME TEMP ºC0 463 4610 5522 7027 7028 46HPLC PROGRAMTIME Na315 % Na740 % RG011 %0 100 0 07.3 100 0 015 53 47 015.1 0 100 028 0 100 028.1 0 0 10030 0 0 100103Temperature: 130 CReagent: Trione Ninhydrin reagentFlow Rate: 0.5 mL/minDetection: UV/VIS 570 nm for primary amino acids440 nm for secondary amino acidsRuntime: 30 minEquilibration Time: 9 min1 23 456 7 8129 10 15111314161718190.0 5.0 10.0 15.0 20.0 25.0minChromatogram of Protein Hydrolysate Standard1 Aspartic Acid2 Threonine3 Serine4 Glutamic Acid5 Proline6 Glycine7 Alanine8 Cystine9 Valine10 Methionine11 Isoleucine12 Leucine13 Tyrosine14 Phenylalanine15 Lysine16 Histidine17 Ammonia18 Tryptophan19 ArgininePRODUCT CATALOGPICKERING LABORATORIES10/11