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METHOD ABSTRACTSAnalytical Conditions: Water/Ace<strong>to</strong>nitrile Pro<strong>to</strong>colColumn: 4.0 x 250 mm, <strong>Pickering</strong> Cat.No. 0840250Sample Injection: 10 µl in MeOH (for all chroma<strong>to</strong>grams shown)Mobile Phase:Solvent A: HPLC-grade WaterSolvent B: HPLC-grade Ace<strong>to</strong>nitrile (ACN)Program:TIME H 2O % ACN % GRADIENT0 90 102 90 10 Isocratic46 49 51 Linear46.1 30 70 Step49 30 70 IsocraticEquilibration Time: 13 minutesEluant Flow Rate: 0.80 mL/minuteColumn Temperature: 37 CReagent 1: 0.3 mL/minute, CB130 Hydrolysis ReagentReagent 2: 0.3 mL/minute, OPA reagent 1 for derivatizationReac<strong>to</strong>rs:Hydrolysis: 100 C, 500 µLDerivatization: Ambient, 100 µLFl<strong>our</strong>escence Detection:Excitation: 330 nmEmission: 465 nmAnalytical Conditions: Water/Methanol Pro<strong>to</strong>colMobile Phase:Solvent A: HPLC-grade WaterSolvent B: HPLC-grade Methanol (MeOH)Program:TIME H 2O % MeOH % GRADIENT0 88 122 88 12 Isocratic42 34 66 Linear46 34 66 Isocratic46.1 0 100 Step49 0 100 Isocratic1131 o-Phthalaldehyde (O120) and Thiofl <strong>our</strong> (3700-2000), <strong>Pickering</strong>’sbrand of Dimethylaminoethanethiol•HCI, in OPA Diluent (CB910)REFERENCE:R.T. Krause, J. Chroma<strong>to</strong>graphy, 185, 615-624 (1979)R.T. J. Chroma<strong>to</strong>graphic Science, 16, 281-288 (1978)H.A. Moye, S.J. Scherer and P.A. St. John, Anal. Letters, 10, 1049-1073 (1977)A. de Kok and M. Hiemstra, J. AOAC int’l, 75, 1063-1072 (1992)Offi cial Methods of Analysis, 15th Ed., AOAC Int’l, Arling<strong>to</strong>n, VA, (291-293)Method 531.1, U.S. Environmental Protection AgencyPRODUCT CATALOGPICKERING LABORATORIES10/11
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