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CHROMATOGRAMS, COLUMNS & GUARDSGLYPHOSATE HERBICIDE ANALYSIS COLUMN<strong>Pickering</strong> Labora<strong>to</strong>ries cation-exchange Glyphosate columnis designed and tested for analysis of Glyphosate and itsprimary metabolite Aminomethylphosphonic acid (AMPA).This short, isocratic method provides separation of thepeaks of interest. Reproducible performance is guaranteedrun-<strong>to</strong>-run and column-<strong>to</strong>-column.Post-column Conditions For Glyphosate Analysis:Reagent 1: Oxidizing reagent—100 µL of 5 % SodiumHypochlorite in 950 mL of GA116Reagent 2: 100 mg of OPA, 2 g Thiouor in 950 mL of GA104Reac<strong>to</strong>r 1: 36 °C, 0.5 mLReac<strong>to</strong>r 2: ambient. 0.1 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmGLYPHOSATE COLUMN 1954150 (4.0 X 150 mm) K+ CATION-EXCHANGE.TO BE USED WITH CATION-EXCHANGE GARD COLUMN PROTECTIONSYSTEM OR GUARD COLUMN (3.0 X 20 mm), K+ CATION-EXCHANGEGlyphosateAMPA0 10 20 minGlyphosate Test Mixture, 2.5 µg/mLCONDITIONSTIME (Min) K200 % RG019 %0 100 015 100 015.1 0 10017 0 10017.1 100 027 100 0FLOW RATE: 0.4 mL/min,COLUMN TEMP.: 55 ºC,INJECTION VOLUME: 10 LGLYPHOSATE COLUMNS CATALOG INFORMATIONCATALOG NO.DESCRIPTION1954150Cation-exchange Column for Glyphosate analysis, 4 × 150 mm &1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL1953020 Cation-exchange Guard Column, 3.0 × 20 mm1952150Micro Cation-exchange Column, K+ form, 2.1 × 150 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL1954250Column, Glyphosate 4 x 250 mm & 1700-0080 Glyphosate testmixture, 2.5 µg/mL, 1.5 mLCATALOG NO.1700-3102GARD COLUMN PROTECTION SYSTEM(FOR USE WITH ANY CATION-EXCHANGE COLUMN)DESCRIPTIONCation-exchange GARD Assembly: Includes holder and2 replaceable GARDS1700-3101 Replacement Cation-exchange GARDs (2/PK)1700-3100 GARD Holder

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