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METHOD ABSTRACTS382 / 70-MIN AMINO ACID ANALYSIS OFPHYSIOLOGICAL SAMPLESPickering Laboratories specializes in the manufacturing of cation-exchangecolumns and eluants for amino acid analysis. Post-column derivatizationwith Ninhydrin offers unmatched selectivity and reproducibility of theanalysis for most challenging matrixes.Pinnacle PCX post-column derivatization system allows analysts to combineeluant gradient capabilities of modern HPLC instruments with columntemperature gradients. We introduce new accelerated Amino Acids Analysismethod for physiological samples that utilizes temperature and eluantgradients.COLUMN OVEN PROGRAMTIME TEMP ºC0 346 3417 6525 7070 7071 341HPLC PROGRAMTIME 1700-1125 % Li365 % Li375 % RG003 %0 100 0 0 010 100 0 0 019 40 60 0 032 0 100 0 043 0 100 0 043.1 0 0 100 057 0 0 100 057.1 0 0 70 3072 0 0 70 3072.1 100 0 0 0METHODAnalytical ConditionsColumn: High-efciency Lithiumcation-exchange column,4.6 x 75 mm, 5 µmCatalog number 0354675TFlow Rate: 0.55 mL/minMobile Phase: 1700-1125, Li365, Li375, RG003Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.5 mLTemperature: 130 CReagent: TrioneFlow Rate: 0.5 mL/minDetection: UV/VIS 570 nm for primary amino acids440 nm for secondary amino acidsRuntime: 72 minEquilibration Time: 15 min4320222357 8 111091315171618 211924232931343335363837394225 2627 28461214:303240 410 10 20 30 40 50 60 70min1 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 -Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 -Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Cystathionine23 Isoleucine24 Leucine25 Tyrosine26 Phenylalanine27 -Alanine28 -Amino-i-butyric acid29 Homocystine30 -Aminobutyric acid31 Tryptophan32 Ethanolamine33 Ammonia34 Hydroxylysines35 Ornitine36 Lysine37 1-Methylhistidine38 Histidine39 3-Methylhistidine40 Anserine41 Carnosine42 Arginine43 Glucosaminic Acid**Internal Standard
MetTyrPheLeu + IleMETHOD ABSTRACTS107 / PKU AND MSUDSCREENING FOR PHENYLKETONURIA AND MAPLE SYRUP URINE DISEASEPhenylketonuria (PKU) and Maple syrup urine disease (MSUD, branched-chain ketoaciduria) are genetically transmittedmetabolic diseases. PKU results in elevated Phenylalanine levels due to decient activity of the enzyme Phenylalaninehydroxylase. In the MSUD state, elevated levels of leucine (Leu), isoleucine (Ile), valine (Val), and alloiso-leucine can bedetected in blood. The increased concentration of the branched-chain amino acids is caused by an inherited decient activityof the enzyme branched-chain keto-acid decarboxylase.Failure to diagnose and treat these conditions in newborns can result in severe mental retardation (PKU) or even death (MSUD).Dietary restriction of Val, Leu, and Ile for MSUD newborns must begin immediately once the diagnosis is made or suspected.MSUD patients must be monitored on a regular schedule in conjunction with the dietary therapy because evenrelatively small changes in the intake of the branched-chain amino acids may produce rather large uctuations of their concentrationsin the plasma. In this respect, MSUD is more difcult to control by dietary means than phenylketonuria (PKU),because each of the branched-chain amino acids has to be individually adjusted and monitored.The Pickering Laboratories PKU/MSUD post-column system is a rapid, automated method for the quantitative analysis ofMet, Leu + Ile, Tyr and Phe from whole blood samples. Between-injection time is 13 min while actual time is only 7 min.The Pickering lithium ion-exchange column has a matched set of eluant and regenerant to provide the best possible separation,sensitivity, and reproducibility. The post-column derivatizing reagent is TRIONE® Ninhydrin.METHODAnalytical ConditionsColumn: PKU/MSUD column, 4.0 x 50 mmCatalog No. 0354050Temperature: 65 °CFlow Rate: 0.4 mL/minMobile Phase: Eluant (A): Li357, Regenerant (B): RG003Gradient:0–8.0 min 100 % A8.1–10.0 min 100 % B10.1–13.0 min 100 % APost-column ConditionsPost-column System: Pinnacle PCXReaction Dwell: 0.5 mLTemperature: 130 °CReagent: TRIONE® NinhydrinFlow Rate: 0.3 mL/minDetection: Visible @ 570 nm01 23Neonate serumwith elevatedPhenylalanineNormalNeonate serum4 5 6 7 8minThis chromatogram is reproduced by courtesy of Quynh Vantu and Wanda Andrews, VirginiaNewborn Screenng Laboratory, Richmond, Virginia.105“TRIONE” (U.S. Patent No. 4,274,833) is a registeredtrademark of Pickering Laboratories, Inc.PRODUCT CATALOGPICKERING LABORATORIES10/11
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TABLE OF CONTENTSINTRODUCTION3 Cust
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INTRODUCTIONINSTALLATION & TRAINING
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“A LOT HAS CHANGED SINCE PRODUCIN
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INSTRUMENTS & HARDWAREFEATURE HIGHL
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INSTRUMENTS & HARDWARE• Indoor us
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INSTRUMENTS & HARDWARECRX400 POST-C
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CHROMATOGRAMS, COLUMNS & GUARDSPick
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CHROMATOGRAMS, COLUMNS & GUARDSREVE
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CHROMATOGRAMS, COLUMNS & GUARDS70-M
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSSODI
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSCARB
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CHROMATOGRAMS, COLUMNS & GUARDSSEPA
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CHROMATOGRAMS, COLUMNS & GUARDSALKI
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CHROMATOGRAMS, COLUMNS & GUARDSCOND
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CHROMATOGRAMS, COLUMNS & GUARDSAFLA
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CHROMATOGRAMS, COLUMNS & GUARDSVOGL
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CHROMATOGRAMS, COLUMNS & GUARDSANAL
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“PURE CHEMICALS DO WHAT THEY ARE
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REAGENTS & CHEMICALSSERAPREP & URIP
Page 56 and 57: REAGENTS & CHEMICALSRESTORECHROMATO
Page 58 and 59: REAGENTS & CHEMICALSCALIBRATION STA
Page 60 and 61: ELUANTSCHROMATOGRAPHIC GRADEPickeri
Page 62 and 63: APPLICATION KITSAmino Acid Analysis
Page 64 and 65: BECKMAN 6300/7300 COLUMNS & CHEMICA
Page 66 and 67: REPLACEMENT COMPONENTS, FITTINGS &
Page 74 and 75: “THE LCTECH STORY”Pickering Lab
Page 76 and 77: LCTECH / GPC PRODUCT LINEGPC ULTRA
Page 78 and 79: LCTECH / GPC PRODUCT LINEUtilizing
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Page 82 and 83: LCTECH / GPC PRODUCT LINEFEATURE HI
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Page 86 and 87: LCTECH / GPC PRODUCT LINE3Intensity
Page 88 and 89: LCTECH / GPC PRODUCT LINEEVA IIICON
Page 90 and 91: LCTECH / MYCOTOXIN PRODUCT LINEUV D
Page 92 and 93: LCTECH / MYCOTOXIN PRODUCT LINESAMP
Page 94 and 95: LCTECH / MYCOTOXIN PRODUCT LINEOTAR
Page 96 and 97: LCTECH / MYCOTOXIN PRODUCT LINEACCE
Page 98 and 99: “CUSTOMERS REALLY ONLY WANT THE C
Page 100 and 101: METHOD ABSTRACTS203.1 / MULTI-RESID
Page 102 and 103: METHOD ABSTRACTS208 / MULTI-RESIDUE
Page 104 and 105: METHOD ABSTRACTS328 / ANALYSIS OF A
Page 108 and 109: METHOD ABSTRACTS205 / AMINOGLYCOSID
Page 110 and 111: METHOD ABSTRACTS102 / ANALYSIS OF B
Page 112 and 113: METHOD ABSTRACTS120 / BROMATE ANALY
Page 114 and 115: METHOD ABSTRACTS108 / POST-COLUMN L
Page 116 and 117: METHOD ABSTRACTS112 / EXPANDED HPLC
Page 118 and 119: METHOD ABSTRACTS325 / ANALYSIS OF F
Page 120 and 121: METHOD ABSTRACTS206 continued from
Page 124 and 125: METHOD ABSTRACTS105.2 / PARALYTIC S
Page 126 and 127: METHOD ABSTRACTS104 / POLYETHER ANT
Page 128 and 129: METHOD ABSTRACTS124 / SULFONAMIDE A
Page 132 and 133: GENERAL TERMS & CONDITIONS1 CONTROL
Page 134: DISTRIBUTORSContinued from previous
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