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METHOD ABSTRACTS208 / MULTI-RESIDUE MYCOTOXIN ANALYSIS OF DRY DISTILLERS GRAINSSINGLE RUN ANALYSIS OF AFLATOXINS, OCHRATOXIN A, ZEARALENONEAND FUMONISINS BY HPLC AND POST-COLUMN DERIVATIZATIONDistillers grains (DG) are the still residues after the ethanol has been collected. Approximately 90 % of US production isused in domestic animal feed. Any Mycotoxins present in the fresh corn can be concentrated by a factor of three. Contaminationcan also occur during storage. This raises concern about the potential animal and human health hazards fromthe use of Mycotoxin contaminated distillers grains. Corn entering the ethanol processing plant as well as distillers grainsshould be routinely tested for Mycotoxin contaminations to ensure compliance with guidelines set by FDA.We present a single screen method to cover 4 families of toxins that could be present in dry distillers grains (DDG).Sample Extraction and Clean-up25 g of nely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of ltered extract isdiluted with 70 mL of Phosphate Buffered Saline (PBS). Aatoxins, Zearealenone and OchratoxinA are isolated using AOZImmunoafnity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are eluted with2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoafnity column (Vicam, USA) according to theprocedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol and combined with eluant fromAOZ column. Solution is evaporated to 0.5 mL and nal volume is adjusted to 1 mL with Methanol.METHODAnalytical ConditionsColumn: MYCOTOX reversed-phase C 18,4.6x250 mm Catalog No. 1612124Temperature: 40 ºCFlow Rate: 1 mL/minMobile Phase: Sodium Phosphate buffer, pH 3.5Catalog No 1700-1108/MeOH/ACNPost-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 1.4 mLTemperature: 60 CReagent: OPA, Thiouor, Brij 35® in GA104Photochemical Reactor: UVEDetection:FluorescenceAatoxins (photochemical derivatization) ex= 365 nm; em= 455 nmFumonisins (post-column derivatization with OPA) ex= 330 nm; em= 465 nmOchratoxin A ex= 335 nm; em= 455 nmZearalenone ex= 275 nm; em= 455 nmREFERENCES:Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thex, N., Multiresidue Mycotoxin Analysisin Corn Grain by Column High-Performance Liquid Chromatography with Post-columnPhotochemical and Chemical Derivatization: Single-Laboratory Validation.(2009) J.AOAC Int., 92, 15-25