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METHOD ABSTRACTS124 / SULFONAMIDE ARTIFICIAL SWEETENERS IN FOOD PRODUCTSANALYSIS OF ACESULFAME, SACCHARIN AND CYCLAMATE BY HPLC WITH POST-COLUMN DERIVATIZATIONNonnutritive sweeteners are widely used in foods and beverages. Since some studies raised questions about long-term safetyof these compounds, their concentration in food products is regulated. Among the articial sweeteners used worldwideSaccharin and Cyclamate cause most controversy and their use is restricted in many countries, including the US. Liquidchromatography is a method of choice for articial sweeteners but Cyclamate analysis is complicated by the fact that thiscompound does not exhibit noticeable UV absorbance.This method allows for simultaneous analysis of Acesulfame, Saccharin and Cyclamate by LC with post-column derivatizationfollowed by uorescence detection. Since the sweeteners are commonly used in combination, a single method ofanalysis is preferred.METHODAnalytical ConditionsAnalytical Column: Reversed-phase column, C18, 2x100 mmTemperature: 50 CFlow Rate: 0.2 mL/minMobile Phase:0.02 M Potassium Phosphate Monobasic in water. Isocraticfor 8 min, followed by washing the column with 70 % ACN– 30 % 0.02 M KH 2PO 4Post-column ConditionsPost-column System: Pinnacle PCXReagent: Hexadecyltrimethyl ammonium bromide (10-3 M),1,6 Diphenyl-1,3,5-hexatriene (4x10-6 M)Reactor: 40 C, 0.1 mLFlow Rate: 0.3 mL/minDetection: Fluorescence, ex= 365 nm, em= 460 nmAcesulfame KSaccharinCyclamate0 2.5 57.5 minChromatograms of a) Diet cola containing Acesulfame K; b) Soft drink spiked with 60 ppm ofartifi cial sweeteners.baREFERENCESJames F. Lawrence, Analyst, vol. 112, No 6 (1987), 879-881James F. Lawrence, Claudette F. Charbonneau, J. Assoc. Anal.Chem., vol. 71, No 5 (1988), 934-937