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CHROMATOGRAMS, COLUMNS & GUARDSALKION COLUMN ANALYSIS OF AMINOGLYCOSIDEANTIBIOTICS IN FEEDSPost-column Conditions:Reagent 1: 300 mg of OPA, 2g Thiouor, 3 mL of 30 %Brij® 35 in 950 mL of OD104Reactor: 45 °C, 0.15 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmApramycinCONDITIONS FOR APRAMYCIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 67 33 05 67 33 015 14.7 7.3 7820 14.7 7.3 7820.1 0 22 7821 0 22 7821.1 67 33 028 67 33 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L05 10 15 20 25 30minGentamicinComplexCONDITIONS FOR GENTAMICIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 43 31 2620 9 13 7830 9 13 7830.1 0 22 7831 0 22 7831.1 43 31 2638 43 31 26FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L05 10 15 20 25 30minNeomycinCONDITIONS FOR NEOMYCIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 015 13.2 8.8 7825 11 11 7825.1 0 22 7826 0 22 7826.1 60 40 032 60 40 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L05 10 15 20 25 30min