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METHOD ABSTRACTS102 / ANALYSIS OF BIOGENIC AMINESFOOD AND PHYSIOLOGICAL SAMPLESHistamine and other polyamines such as Putrescine, Cadaverine, Spermidine and Spermine are products of decomposition.The level of histamine, in particular, has been used as a regulatory guideline for degree of decomposition in sh. Also,Histamine and Tyramine levels are of interest to producers of wine and cheese.The polyamines, Spermidine, Spermine and Putrescine are elevated in neoplastic and damaged, e.g., burned tissue. In fact,the level of circulating and urinary polyamines is an indicator of total body damage. The levels can be monitored for theeffect of diet and other modalities on recovery.This method employs ion-exchange chromatography so, after extraction, centrifugation/ltration are the only samplepreparations necessary.Extraction ProcedureWeigh 10 g of sample into a small glass blender cup (anyfortications should be added at this time). Add 50 mL ofextraction solution, 80 % HPLC Methanol and 20 % 0.1N Hcl, and homogenize for two minutes. Centrifuge forve minutes. Mix equal portions of the supernatant withthe mobile phase (K600). Allow to coagulate at -4 °C for 15minutes, then centrifuge for ve minutes. The clear supernatantis ltered (0.45 µm Nylon) and placed in an autosamplervial.METHODAnalytical ConditionsColumn: ALKION cation-exchange, K+ form,4 x 150 mm, Catalog No. 9410917ALKION Guard column, 3 x 20 mm,Catalog No. 9493020Temperature: 45 °CFlow Rate: 0.8 mL/minMobile Phase: K600, K563, K130Post-column ConditionsPost-Column System: Pinnacle PCXReactor Volume: 0.15 mLTemperature: 45 °CReagent: 300 mg of OPA, 2 g of Thiouor,3 mL of 30 % Brij® 35 in 950 mL of OD104Flow Rate: 0.3 mL/minDetection: Fluorometer ex: 330 nm, em: 465 nm
METHOD ABSTRACTSHistamine5 Days Old5 Days OldFreshPutrescineCadaverineHistamineFreshPutrescineCadaverineTIME (Min) K600 % K130 %0 100 012 100 012.1 0 10015 0 10015.1 100 020 100 002 4 6 8 10Ahi Tunamin02 4 6 8 10Mahi-Mahimin1 Histamine2 Putrescine3 Cadaverine1093 425015 10 15 20minGradient Separation of Polyamine MixtureTIME (Min) K600 % K563 % K130 %0 100 0 06 100 0 015 0 100 021 0 100 021.1 0 0 10023 0 0 10023.1 100 0 029 100 0 01 Histamine2 Putrescine3 Cavaverine4 Spermidine5 SperminePRODUCT CATALOGPICKERING LABORATORIES10/11
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TABLE OF CONTENTSINTRODUCTION3 Cust
Page 6 and 7:
INTRODUCTIONINSTALLATION & TRAINING
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“A LOT HAS CHANGED SINCE PRODUCIN
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INSTRUMENTS & HARDWAREFEATURE HIGHL
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INSTRUMENTS & HARDWARE• Indoor us
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INSTRUMENTS & HARDWARECRX400 POST-C
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CHROMATOGRAMS, COLUMNS & GUARDSPick
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CHROMATOGRAMS, COLUMNS & GUARDSREVE
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CHROMATOGRAMS, COLUMNS & GUARDS70-M
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSSODI
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSCARB
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CHROMATOGRAMS, COLUMNS & GUARDSSEPA
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CHROMATOGRAMS, COLUMNS & GUARDSALKI
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CHROMATOGRAMS, COLUMNS & GUARDSCOND
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CHROMATOGRAMS, COLUMNS & GUARDSAFLA
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CHROMATOGRAMS, COLUMNS & GUARDSVOGL
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CHROMATOGRAMS, COLUMNS & GUARDSANAL
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“PURE CHEMICALS DO WHAT THEY ARE
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REAGENTS & CHEMICALSSERAPREP & URIP
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REAGENTS & CHEMICALSRESTORECHROMATO
Page 58 and 59:
REAGENTS & CHEMICALSCALIBRATION STA
Page 60 and 61: ELUANTSCHROMATOGRAPHIC GRADEPickeri
Page 62 and 63: APPLICATION KITSAmino Acid Analysis
Page 64 and 65: BECKMAN 6300/7300 COLUMNS & CHEMICA
Page 66 and 67: REPLACEMENT COMPONENTS, FITTINGS &
Page 74 and 75: “THE LCTECH STORY”Pickering Lab
Page 76 and 77: LCTECH / GPC PRODUCT LINEGPC ULTRA
Page 78 and 79: LCTECH / GPC PRODUCT LINEUtilizing
Page 80 and 81: LCTECH / GPC PRODUCT LINE• Can be
Page 82 and 83: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 84 and 85: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 86 and 87: LCTECH / GPC PRODUCT LINE3Intensity
Page 88 and 89: LCTECH / GPC PRODUCT LINEEVA IIICON
Page 90 and 91: LCTECH / MYCOTOXIN PRODUCT LINEUV D
Page 92 and 93: LCTECH / MYCOTOXIN PRODUCT LINESAMP
Page 94 and 95: LCTECH / MYCOTOXIN PRODUCT LINEOTAR
Page 96 and 97: LCTECH / MYCOTOXIN PRODUCT LINEACCE
Page 98 and 99: “CUSTOMERS REALLY ONLY WANT THE C
Page 100 and 101: METHOD ABSTRACTS203.1 / MULTI-RESID
Page 102 and 103: METHOD ABSTRACTS208 / MULTI-RESIDUE
Page 104 and 105: METHOD ABSTRACTS328 / ANALYSIS OF A
Page 106 and 107: METHOD ABSTRACTS382 / 70-MIN AMINO
Page 108 and 109: METHOD ABSTRACTS205 / AMINOGLYCOSID
Page 112 and 113: METHOD ABSTRACTS120 / BROMATE ANALY
Page 114 and 115: METHOD ABSTRACTS108 / POST-COLUMN L
Page 116 and 117: METHOD ABSTRACTS112 / EXPANDED HPLC
Page 118 and 119: METHOD ABSTRACTS325 / ANALYSIS OF F
Page 120 and 121: METHOD ABSTRACTS206 continued from
Page 124 and 125: METHOD ABSTRACTS105.2 / PARALYTIC S
Page 126 and 127: METHOD ABSTRACTS104 / POLYETHER ANT
Page 128 and 129: METHOD ABSTRACTS124 / SULFONAMIDE A
Page 132 and 133: GENERAL TERMS & CONDITIONS1 CONTROL
Page 134: DISTRIBUTORSContinued from previous
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