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METHOD ABSTRACTS208 / MULTI-RESIDUE MYCOTOXIN ANALYSIS OF DRY DISTILLERS GRAINSSINGLE RUN ANALYSIS OF AFLATOXINS, OCHRATOXIN A, ZEARALENONEAND FUMONISINS BY HPLC AND POST-COLUMN DERIVATIZATIONDistillers grains (DG) are the still residues after the ethanol has been collected. Approximately 90 % of US production isused in domestic animal feed. Any Mycotoxins present in the fresh corn can be concentrated by a factor of three. Contaminationcan also occur during storage. This raises concern about the potential animal and human health hazards fromthe use of Mycotoxin contaminated distillers grains. Corn entering the ethanol processing plant as well as distillers grainsshould be routinely tested for Mycotoxin contaminations to ensure compliance with guidelines set by FDA.We present a single screen method to cover 4 families of toxins that could be present in dry distillers grains (DDG).Sample Extraction and Clean-up25 g of nely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of ltered extract isdiluted with 70 mL of Phosphate Buffered Saline (PBS). Aatoxins, Zearealenone and OchratoxinA are isolated using AOZImmunoafnity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are eluted with2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoafnity column (Vicam, USA) according to theprocedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol and combined with eluant fromAOZ column. Solution is evaporated to 0.5 mL and nal volume is adjusted to 1 mL with Methanol.METHODAnalytical ConditionsColumn: MYCOTOX reversed-phase C 18,4.6x250 mm Catalog No. 1612124Temperature: 40 ºCFlow Rate: 1 mL/minMobile Phase: Sodium Phosphate buffer, pH 3.5Catalog No 1700-1108/MeOH/ACNPost-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 1.4 mLTemperature: 60 CReagent: OPA, Thiouor, Brij 35® in GA104Photochemical Reactor: UVEDetection:FluorescenceAatoxins (photochemical derivatization) ex= 365 nm; em= 455 nmFumonisins (post-column derivatization with OPA) ex= 330 nm; em= 465 nmOchratoxin A ex= 335 nm; em= 455 nmZearalenone ex= 275 nm; em= 455 nmREFERENCES:Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thex, N., Multiresidue Mycotoxin Analysisin Corn Grain by Column High-Performance Liquid Chromatography with Post-columnPhotochemical and Chemical Derivatization: Single-Laboratory Validation.(2009) J.AOAC Int., 92, 15-25
METHOD ABSTRACTSFB2FB1Mycotoxin StandardOPA onOPA offOPA onOchraG1G2 B2B1Zea0 10 20 30 40 50minMYCOTOXINSPIKECONC, ppbNATURAL CONTAMINATIONLEVEL, ppbRECOVERIES % RSD N=4, %Aflatoxin B1 10.0 65 7.6Aflatoxin B2 3.4 79 6.3Aflatoxin G1 10.2 75 9.4Aflatoxin G2 4.4 82 9.1Ochratoxin A 203 89 7.1Zearalenone 1057 231 75 8.8Fumonisin B1 1042 801 109 5.8Fumonisin B2 1379 223 104 6.8101DDG Sample Spiked with MycotoxinsOPA onFB1FB2OPA offOPA onG2G1B2 B1OchraZea0 10 20 30 40 50min5-POINT CALIBRATION CURVESMYCOTOXIN CONCENTRATION RANGE CORRELATIONAflatoxin B1 0.23 – 113.1 ppb 0.99926Aflatoxin B2 0.2 – 39.7 ppb 0.99966Aflatoxin G1 0.5 – 58.2 ppb 0.99933Aflatoxin G2 0.2 – 24.7 ppb 0.99941Ochratoxin A 9.2 – 1155 ppb 0.99926Zearalenone 0.024 – 12.01 ppm 0.99908Fumonisin FB1 0.024 – 11.84 ppm 0.99987Fumonisin FB2 0.031 – 7.84 ppm 0.99993PRODUCT CATALOGPICKERING LABORATORIES10/11
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TABLE OF CONTENTSINTRODUCTION3 Cust
Page 6 and 7:
INTRODUCTIONINSTALLATION & TRAINING
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“A LOT HAS CHANGED SINCE PRODUCIN
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INSTRUMENTS & HARDWAREFEATURE HIGHL
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INSTRUMENTS & HARDWARE• Indoor us
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INSTRUMENTS & HARDWARECRX400 POST-C
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CHROMATOGRAMS, COLUMNS & GUARDSPick
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CHROMATOGRAMS, COLUMNS & GUARDSREVE
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CHROMATOGRAMS, COLUMNS & GUARDS70-M
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSSODI
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSCARB
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CHROMATOGRAMS, COLUMNS & GUARDSSEPA
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CHROMATOGRAMS, COLUMNS & GUARDSALKI
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CHROMATOGRAMS, COLUMNS & GUARDSCOND
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CHROMATOGRAMS, COLUMNS & GUARDSAFLA
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CHROMATOGRAMS, COLUMNS & GUARDSVOGL
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CHROMATOGRAMS, COLUMNS & GUARDSANAL
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“PURE CHEMICALS DO WHAT THEY ARE
Page 54 and 55: REAGENTS & CHEMICALSSERAPREP & URIP
Page 56 and 57: REAGENTS & CHEMICALSRESTORECHROMATO
Page 58 and 59: REAGENTS & CHEMICALSCALIBRATION STA
Page 60 and 61: ELUANTSCHROMATOGRAPHIC GRADEPickeri
Page 62 and 63: APPLICATION KITSAmino Acid Analysis
Page 64 and 65: BECKMAN 6300/7300 COLUMNS & CHEMICA
Page 66 and 67: REPLACEMENT COMPONENTS, FITTINGS &
Page 74 and 75: “THE LCTECH STORY”Pickering Lab
Page 76 and 77: LCTECH / GPC PRODUCT LINEGPC ULTRA
Page 78 and 79: LCTECH / GPC PRODUCT LINEUtilizing
Page 80 and 81: LCTECH / GPC PRODUCT LINE• Can be
Page 82 and 83: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 84 and 85: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 86 and 87: LCTECH / GPC PRODUCT LINE3Intensity
Page 88 and 89: LCTECH / GPC PRODUCT LINEEVA IIICON
Page 90 and 91: LCTECH / MYCOTOXIN PRODUCT LINEUV D
Page 92 and 93: LCTECH / MYCOTOXIN PRODUCT LINESAMP
Page 94 and 95: LCTECH / MYCOTOXIN PRODUCT LINEOTAR
Page 96 and 97: LCTECH / MYCOTOXIN PRODUCT LINEACCE
Page 98 and 99: “CUSTOMERS REALLY ONLY WANT THE C
Page 100 and 101: METHOD ABSTRACTS203.1 / MULTI-RESID
Page 104 and 105: METHOD ABSTRACTS328 / ANALYSIS OF A
Page 106 and 107: METHOD ABSTRACTS382 / 70-MIN AMINO
Page 108 and 109: METHOD ABSTRACTS205 / AMINOGLYCOSID
Page 110 and 111: METHOD ABSTRACTS102 / ANALYSIS OF B
Page 112 and 113: METHOD ABSTRACTS120 / BROMATE ANALY
Page 114 and 115: METHOD ABSTRACTS108 / POST-COLUMN L
Page 116 and 117: METHOD ABSTRACTS112 / EXPANDED HPLC
Page 118 and 119: METHOD ABSTRACTS325 / ANALYSIS OF F
Page 120 and 121: METHOD ABSTRACTS206 continued from
Page 124 and 125: METHOD ABSTRACTS105.2 / PARALYTIC S
Page 126 and 127: METHOD ABSTRACTS104 / POLYETHER ANT
Page 128 and 129: METHOD ABSTRACTS124 / SULFONAMIDE A
Page 132 and 133: GENERAL TERMS & CONDITIONS1 CONTROL
Page 134: DISTRIBUTORSContinued from previous
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