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CHROMATOGRAMS, COLUMNS & GUARDSPOLYETHER ANTIBIOTICS ANALYSIS COLUMNPolyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid supplements andnal feeds, are best quantied using HPLC with post-column derivatization. Because of selectivity of post-column reaction,almost no sample clean-up is needed.LC Conditions:Flow Rate: 0.7 mL/min, column temperature 40 °C,injection volume 10 µLMobile Phase: Methanol/5 % Acetic Acid inWater (90:10), isocraticFeed Extract2Post-column Conditions(non-metallic post-column derivatization system is required):Reagent 1: Concentrated Sulfuric acid / Methanol (4:96 v/v)Reactor 1: Ambient, 0.1 mLReagent 2: 60 g of Vanillin (or p-dimethylaminobenzaldehyde)in 950 mL of MethanolReactor 2: 90 °C, 1.4 mLReagents Flow Rate: 0.3 mL/minNOTE: Using 2-reagent system for this application greatly extends reagents life.One-reagent method and post-column derivatizationsystem are also available.Detection:UV-Vis detector: Vanillin =520 nm, DMAB =450 nm14323Standard10510 15min1 Monensin B 2 Monensin A 3 Narasin APOLYETHER ANTIBIOTICS CATALOG INFORMATIONCATALOG NO.DESCRIPTION2381750 Polyether reversed-phase column, C 18, 4.6 × 250 mm18ECG001 Guard Cartridge Holder with 3 cartridges18ECG002 Guard Cartridges, 2/pk.PRODUCT CATALOGPICKERING LABORATORIES10/11