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METHOD ABSTRACTS325 / ANALYSIS OF FORMALDEHYDESIMPLE AND SENSITIVE METHOD FOR ANALYZING FREE FORMALDEHYDEFormaldehyde is used for disinfections of industrially manufactured products. However, it is detrimental to health and itspotential oncogenicity led to the current utilization of a formaldehyde donor for long-term preservation. Such donors are inhydrolytic equilibrium with formaldehyde producing free formaldehyde on exposure to moisture.This method is used to quantify the free formaldehyde without upsetting the existing equilibrium. Potential interferencesare either separated from formaldehyde or they don’t react with the post-column reagent. The lutidine derivative is highlyuorescent resulting in ppm detection levels.METHODAnalytical ConditionsColumn: Diol, 5 µm, 4.6 x 250 mmTemperature: 40 CFlow Rate: 0.5 mL/minMobil Phase: Acetonitrile: Water (85:15) isocraticPost-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.5 mLTemperature: 100 CReagent:0.81 M Ammonimn acetate0.12 M Galcial acetic acid (pH=5)0.05 M 2, 4-Pentanedione with waterFlow Rate: 0.4 mL/minDetection: Fluorometer ex: 412 nm, em: 510 nm4.0 6.0 8.0 10.0 12.0 14.0 16.0
METHOD ABSTRACTS206 / GLYPHOSATE AND AMPA ANALYSIS IN CROPSA SIMPLE AND REPRODUCIBLE EXTRACTION AND CLEAN-UP 2 FOR HPLC POST-COLUMN DERIVATIZATIONThe recently practiced method1 for analysis of Glyphosate and AMPA in crops suffers from an expensive, time consumingclean-up procedure that has less than ideal recoveries. Although the analysis (after clean-up) by ion-exchange chromatographywith post-column derivatization is rugged and sensitive, a new method was sought to improve the sample preparation.This resulted in AOAC Method 2000.52 2 which has a streamlined clean-up followed by pre-column derivatization andGC/MS analysis. We show how this simplied sample preparation is suitable for the classic ion-exchange/post-columnanalytical protocol.METHODEquipment:• LC with a binary pump• Fluorescence detector• Pickering Laboratories Vector PCX or Pinnacle PCX Post-columnDerivatization instrument• Pickering Laboratories Potassium cation exchange column,4.0 x 150 mm (Cat. No. 1954150)• Pickering Laboratories Potassium cation exchange guard column,3.0 x 20 mm (Cat. No 1953020)• Cation exchange clean-up column (Cat. No 1705-0001)Reagents:• Potassium eluant (Cat. No.K200)• Potassium regenerant (Cat. No. RG019)• Hypochlorite diluent (Cat. No. GA116)• o-Phthalaldehyde diluent (Cat. No. GA104)• Thiofluor (Cat. No. 3700-2000)• o-Phthalaldehyde (Cat. No. O120)• 5 % Sodium hypochlorite solution• Methylene chloride• Acidic modifier solution• Clean-up column eluantSample PreparationExtraction:To 25 g of a homogenous sample add enough water (afterestimation of moisture content) to make the total volume ofwater 125 mL. Blend at high speed for 3–5 min. and centrifugefor 10 min. Transfer 20 mL of the aqueous extract intoa centrifuge tube and add 15 mL of methylene chloride (toremove nonpolar co-extractives). Shake for 2–3 min. andcentrifuge for 10 min. Transfer 4.5 mL of the aqueous layerinto a vial and add 0.50 mL acidic modier solution (16gKH 2PO 4, 160 mL H 2O, 40 mL Methanol, 13.4 mL HCl).Shake and centrifuge for 10 min.Matrix Speci c Modi cation:Plants with high: 1) Water 2) Protein 3) Fat Content1 For crops that absorb large amounts of water, reduce testportion to 12.5 g keeping water volume the same.2 For crops that have high protein content add 100 µL HCl to20 mL aliquot of crude extract. Cap, shake and centrifugefor 10 min.3 For crops that have high oil content, do the methylenechloride partition twice.Cation-Exchange Clean-up:Transfer 1 mL of extract (representing 0.18 g normal cropor 0.09 g dry crop) to the column reservoir and elute to thetop of the resin bed. Add 0.70 mL of the elution solution(160 mL H 20, 2.7 mL HCl, 40 mL Methanol) and discardthe efuent. Repeat with a second 0.70 mL portion anddiscard efuent. Elute with 12 mL of the elution solutionand collect in a round-bottomed ask. Evaporate to drynessin a water bath set at 40 C using a rotary evaporator. Orcollect in a centrifuge tube and evaporate using a vacuumvortex evaporator. Dissolve residue in 2.0 mL of the elutionsolution (use 1.5 mL for dry crops). Extracts before evaporationcan be stored refrigerated for up to 7 days.117Continued on next pagePRODUCT CATALOGPICKERING LABORATORIES10/11
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TABLE OF CONTENTSINTRODUCTION3 Cust
Page 6 and 7:
INTRODUCTIONINSTALLATION & TRAINING
Page 8 and 9:
“A LOT HAS CHANGED SINCE PRODUCIN
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INSTRUMENTS & HARDWAREFEATURE HIGHL
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INSTRUMENTS & HARDWARE• Indoor us
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INSTRUMENTS & HARDWARECRX400 POST-C
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CHROMATOGRAMS, COLUMNS & GUARDSPick
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CHROMATOGRAMS, COLUMNS & GUARDSREVE
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CHROMATOGRAMS, COLUMNS & GUARDS70-M
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSSODI
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CHROMATOGRAMS, COLUMNS & GUARDSHIGH
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CHROMATOGRAMS, COLUMNS & GUARDSSTAN
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CHROMATOGRAMS, COLUMNS & GUARDSCARB
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CHROMATOGRAMS, COLUMNS & GUARDSSEPA
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CHROMATOGRAMS, COLUMNS & GUARDSALKI
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CHROMATOGRAMS, COLUMNS & GUARDSCOND
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CHROMATOGRAMS, COLUMNS & GUARDSAFLA
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CHROMATOGRAMS, COLUMNS & GUARDSVOGL
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CHROMATOGRAMS, COLUMNS & GUARDSANAL
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“PURE CHEMICALS DO WHAT THEY ARE
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REAGENTS & CHEMICALSSERAPREP & URIP
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REAGENTS & CHEMICALSRESTORECHROMATO
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REAGENTS & CHEMICALSCALIBRATION STA
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ELUANTSCHROMATOGRAPHIC GRADEPickeri
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APPLICATION KITSAmino Acid Analysis
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BECKMAN 6300/7300 COLUMNS & CHEMICA
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REPLACEMENT COMPONENTS, FITTINGS &
Page 68 and 69: REPLACEMENT COMPONENTS, FITTINGS &
Page 74 and 75: “THE LCTECH STORY”Pickering Lab
Page 76 and 77: LCTECH / GPC PRODUCT LINEGPC ULTRA
Page 78 and 79: LCTECH / GPC PRODUCT LINEUtilizing
Page 80 and 81: LCTECH / GPC PRODUCT LINE• Can be
Page 82 and 83: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 84 and 85: LCTECH / GPC PRODUCT LINEFEATURE HI
Page 86 and 87: LCTECH / GPC PRODUCT LINE3Intensity
Page 88 and 89: LCTECH / GPC PRODUCT LINEEVA IIICON
Page 90 and 91: LCTECH / MYCOTOXIN PRODUCT LINEUV D
Page 92 and 93: LCTECH / MYCOTOXIN PRODUCT LINESAMP
Page 94 and 95: LCTECH / MYCOTOXIN PRODUCT LINEOTAR
Page 96 and 97: LCTECH / MYCOTOXIN PRODUCT LINEACCE
Page 98 and 99: “CUSTOMERS REALLY ONLY WANT THE C
Page 100 and 101: METHOD ABSTRACTS203.1 / MULTI-RESID
Page 102 and 103: METHOD ABSTRACTS208 / MULTI-RESIDUE
Page 104 and 105: METHOD ABSTRACTS328 / ANALYSIS OF A
Page 106 and 107: METHOD ABSTRACTS382 / 70-MIN AMINO
Page 108 and 109: METHOD ABSTRACTS205 / AMINOGLYCOSID
Page 110 and 111: METHOD ABSTRACTS102 / ANALYSIS OF B
Page 112 and 113: METHOD ABSTRACTS120 / BROMATE ANALY
Page 114 and 115: METHOD ABSTRACTS108 / POST-COLUMN L
Page 116 and 117: METHOD ABSTRACTS112 / EXPANDED HPLC
Page 120 and 121: METHOD ABSTRACTS206 continued from
Page 124 and 125: METHOD ABSTRACTS105.2 / PARALYTIC S
Page 126 and 127: METHOD ABSTRACTS104 / POLYETHER ANT
Page 128 and 129: METHOD ABSTRACTS124 / SULFONAMIDE A
Page 132 and 133: GENERAL TERMS & CONDITIONS1 CONTROL
Page 134: DISTRIBUTORSContinued from previous
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