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CHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY SODIUM CATION-EXCHANGE COLUMN (4.0 X 150 mm)CATALOG NUMBER 1154150USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1193020 (3.0 X 20 mm)USE FOR PROTEIN HYDROLYSATE, COLLAGEN HYDROLYSATETEMPERATURE ISOCRATIC1053 491112131418208Protein Hydrolysate615 1619222175349811121314182010Collagen Hydrolysate161516 1719222705 10 15 20 25 30 35 40 45 50 55 60min1 Methionine-D,L,-Sulfoxide2 trans-4-Hydroxy-L-Proline3 Aspartic Acid4 Threonine5 Serine6 Glutamic Acid7 Proline8 Glycine9 Alanine10 Cystine11 Valine12 Methionine13 Isoleucine14 Leucine15 Tyrosine16 Phenylalanine17 D,L & allo-Hydroxylysine18 Lysine19 Ammonia20 Histidine21 Tryptophan22 ArginineCONDITIONSTIME (Min) 1700-0112 % Na740 % RG011 %0 100 0 012 100 0 034 0 100 053 0 100 053.1 0 0 10055 0 0 10055.1 100 0 067 100 0 0FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 48 ºC,INJECTION VOLUME: 10 L OF 0.25 mole/mL STD.