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CHROMATOGRAMS, COLUMNS & GUARDSCARBAMATE PESTICIDE ANALYSIS COLUMNSPickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues, specied byEPA and AOAC methods. For C 18columns, two water/methanol gradient conditions are available for analysis of methanolicand water samples. Expanded resolution C 8column is capable of separating as many as 23 carbamates with eitherwater/methanol or water/acetonitrile gradients. The difference in selectivity between Methanol and Acetonitrile protocolscould be used for conrming peak identication.Post-column conditions for pesticide analysis:Reagent 1: Hydrolysis reagent CB130 or CB130.2Reagent 2: 100 mg of OPA, 2 g Thiouorin 950 mL of CB910Reactor 1: 100 °C, 0.5 mLReactor 2: ambient. 0.1 mLReagents ow rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmThe recommended gradient conditions are subject tochange without notice. This may happen because of lotchanges in the columns, or improvements in the overallmethod.The recommended gradient for the column will alwaysbe included in the column package, and it supersedes theinformation in this catalog.CARBAMATE COLUMN 1846250 (4.6 X 250 mm), C 18 , 5 µm1423654 ng of Carbamates in 150 µL91178 12100 5 10 15 20 25 30 35 40 45minCONDITIONS FORAQUEOUS SAMPLESTIME (Min) WATER % MeOH %0 100 01 100 01.1 82 1836 30 7039 30 7039.1 0 10041 0 10041.1 100 055 100 0FLOW RATE: 1 mL/min,COLUMN TEMP.: 42 ºC,INJECTION VOLUME: Up to 400 L05 10 15 20 25 30 35 40 45 501 Aldicarb sulfoxide2 Aldicarb sulfone3 Oxamyl4 Methomyl123455 3-Hydroxycarbofuran6 Aldicarb7 Propoxur8 Carbofuran625 ng of Carbamates in 10 µL7891011 12min9 Carbaryl10 1-Naphthol11 Methiocarb12 BDMC (internal standard)CONDITIONS FORMETHANOLIC SAMPLESTIME (Min) WATER % MeOH %0 85 151 85 1544 25 7544.1 0 10049 0 10049.1 85 1557 85 15FLOW RATE: 1 mL/min,COLUMN TEMP.: 42 ºC,INJECTION VOLUME: 10 L