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REAGENTS & CHEMICALSp-DIMETHYLAMINOBENZALDEHYDE (DMAB)CHROMATOGRAPHIC GRADE FOR POST-COLUMN ANALYSISOF SULFA DRUGS IN FEED AND ANIMAL TISSUESp-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent)reacts rapidly with many primary amines to form acomplex with maximum absorbance at 450 nm.Reversed-phase LC followed by post-column derivatizationis used to determine the levels of Sulfamethazine,Sulfathiazole and related compounds in feeds, premixesand animal tissues. Although these compounds have a UVchromophore, so do most of the other constituents in thecomplex sample matrix. Forming a colored derivative postcolumnallows shifting of the detection wavelength into thevisible range thus greatly enhancing selectivity.p-DIMETHYLAMINOBENZALDEHYDE (DMAB) CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY3700-0400 p-Dimethylaminobenzaldehyde (DMAB) each (5 g/bottle)THIOFLUORN,N-DIMETHYL-2-MERCAPTOETHYLAMINE HYDROCHLORIDE – CHROMATOGRAPHIC GRADEFOR AUTOMATED POST-COLUMN DERIVATIZATION OF PRIMARY AMINESPrimary amines form highly uorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptanunder basic conditions.The products of this reaction, 1-alkyl-2-thioalkyl-subsitituted isoindoles, exhibit optimal excitation at 330 nm andmaximal emission at 465 nm.Pickering’s Thiouor, a solid, nearly odorless nucleophile, is a superior substitute for 2-Mercaptoethanol in the preparationof OPA reagents. It forms a more stable reagent and a longer-lasting uorophore with OPA than does 2-Mercaptoethanol, yetit has the same uorescence properties.Unlike the volatile 2-Mercaptoethanol, Thiouor will not migrate through the gas manifold and regulator of the OPAreagent pressurization system.Two grams of Thiouor is equivalent to 1 mL of 2-Mercaptoethanol.51THIOFLUOR CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY3700-2000 Thiofluor each (10 g/bottle)o-PHTHALALDEHYDE (OPA) DILUENTCHROMATOGRAPHIC GRADE FOR PREPARATION OF o-PHTHALALDEHYDE REAGENTSThe derivatization of primary amines with o-Phthalaldehyde and a mercaptan requires basic conditions. To ensure theoptimum pH of the reaction it is important to use the correct diluent.Three application specic diluents are available : CB910 and GA104 for carbamate and glyphosate analyses respectively, andOD104 for analysis of amino acids and other amines.These borate buffers are produced from starting materials which are free of heavy metals and amines. As with most otherproducts of Pickering Laboratories, the quality of the OPA Diluents is veried by actual post-column HPLC analysis.o-PHTHALALDEHYDE (OPA) DILUENT CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYCB910 o-Phthalaldehyde (OPA) Diluent for Carbamate analysis case of 4 (950 mL/bottle)GA104 o-Phthalaldehyde (OPA) Diluent for Glyphosate analysis case of 4 (950 mL/bottle)OD104 o-Phthalaldehyde (OPA) Diluent for amino acids and amines analysis case of 4 (950 mL/bottle)PRODUCT CATALOGPICKERING LABORATORIES10/11