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CHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.0 X 100 mm)CATALOG NUMBER 0354100TUSE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 035202 (2.0 X 20 mm)USE FOR PHYSIOLOGICAL SAMPLES AND COMPLEX MATRICESTEMPERATURE ISOCRATIC1 244351715 16 2018227 8 9 10 11 1213461419212324 2526 27302928313233 34 353637383940414243450 25 5075 1001 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Cystathionine23 Isoleucine24 Leucine25 Norleucine26 Tyrosine27 Phenylalanine28 Alanine29 Amino-i-butyric acid30 Homocystine31 Aminobutyric acid32 Tryptophan33 Ethanolamine34 Hydroxylysines35 Ammonia36 Creatinine37 Ornithine38 Lysine39 Histidine40 3-Methyl-histidine41 1-Methyl-histidine42 Carnosine43 Anserine44 Amino guanidinopropionic acid45 ArginineCONDITIONSSTEP TIME (Min) INTERVAL Li275 % Li750 % RG003 % COMMENT0 0 0 100 0 0 Inject1 8 8 100 0 0 Isocratic2 46 38 65 35 0 Linear Gradient3 86 40 0 100 0 Linear Gradient4 90 4 0 100 0 Isocratic5 115 25 0 94 6 Linear Gradient6 122 2 0 94 6 Isocratic7 122.1 0.1 100 0 0 Step Change8 140 17.9 100 0 0 Re-equilibrationFLOW RATE: 0.35 mL/min, COLUMN TEMP: 37 ºC,INJECTION VOLUME: 10 µL of 0.25 µmole/mL Std.