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METHOD ABSTRACTS206 continued from previous pageLC ConditionsLC Column Temperature: 55 CSample Injection Volume: 100 µLLC Flow Rate: 0.40 mL/minMobile Phases:K200, Potassium EluantRG019, RegenerantPost-column ConditionsPost-column System: Vector PCX or Pinnacle PCXReactor Volume: 0.5 mLReactor Temperature: 36 CReagent 1: 100µL of 5 % NaOCl (Bleach) in GA116 DiluentReagent 2: 100 mg o-Phthalaldehyde and 2g Thiouor in950 mL GA104 DiluentFlow rate: 0.3 mL/minDetection: Fluorescence detector ex: 330 nm, em: 465 nmACKNOWLEDGEMENT:We thank The Montana Department of Agriculture, Laboratory Bureau for extractingalfalfa, tomato and other samples.REFERENCES:1) “Validation of an Analytical Residue Method for Analysis of Glyphosate and Metabolite:An Interlaboratory Study.” J. Agric. Food Chem. 34, (1986) 955–960.2) P.L. Alferness and L.A. Wiebe, “Determination of Glyphosate and AminomethylphosphonicAcid in Crops by Capillary Gas Chromatography with Mass-Selective Detection:Collaborative Study.” Journal of AOAC International, 2001 84, 823–846.STEP TIME (Min) INTERVAL (Min) K200 % RG019 %0 0 0 100 0 Injection1 0-15 15 100 0 Isocratic2 15.1-17 1.9 0 100 Step Change3 17.1-25 7.9 100 0 Re-EquilibrationNOTE: Ensure that the auto sampler injection valve is fi tted with a Tefzel or PEEK rotorseal for compatibility with the high-pH column regenerant.Heavy-metal ions, especially iron can cause loss of sensitivity. Contamination of the guardand column can be removed by using RESTORE (Cat. No. 1700-0140) reagent.GlyphosateAMPAAlfalfa spiked with0.2 ppm glyphosateTomato spiked with0.15 ppm glyphosate2 4 6 8 10 12 14 16 18 20 22 24Time [min]Chromatograms of Alfalfa and Tomato matrix spiked with glyphosate and AMPA