th  - 1987 - 51st ENC Conference

MK17
STRUCTURES OF DNA OLIGOMERS DETERMINED
BY 2D NMR AND DISTANCE GEOMETRY TECHNIQUES.
C~uan Wang* and Arthur Pardi
Department of Chemistry
Rutgers, The State University of New Jersey
New Brunswick, NJ 08903
The double stranded decamers d(~AATC'~) and d(GCGCGTGACG) have been
investigated by 2D NMR. Three-dimensional smlcmres of these molecules were generated by
distance geometry techniques using proton-proton internuclear distances derived from NOESY
spectra. Proton resonance assignments were made for all nonexchangeable aromatic and C1'-C3'
sugar protons, most exchangeable imino and cytosine amino protons, and many C4' and C5' sugar
protons in these molecules. In both decamers more than 40 inter-base pair and 50 intra-base pair
proton-proton distances were calculated fi'om build-up rates derived from NOESY spectra at multiple
mixing times. This distance information was then used as input for a distance geometry program to
generate three-dimensional structures of the oligomers in solution. Correlations between base
sequence and local variations in the DNA smJctme will be discussed.