th  - 1987 - 51st ENC Conference

More documents

Recommendations

Info

Monday - AM Protein Dynamics and Foldlng Studied by Magnetization Transfer NMR Christopher M. Dobson Inorganic Chemistry Laboratory University of Oxford South Parks Road Oxford OXI 3QR England NMR provides an experimental basis for investigating in considerable detall the structures and dynamics of biological molecules in solutlon. As part of our studies of the folding and unfolding of globular proteins, we have been explolting magnetization transfer processes in both one- and two-dlmenslonal NMR experiments. The procedures have been establlshed in studies of lysozyme, where accurate rate constants for both determined by following the time saturation transfer experlments I. effects in two-dlmensional NOESY folding and unfolding have been developments of one-dlmenslonal In addition, chemical exchange experiments have permitted the correlation of resonances in folded and unfolded states, permitting assignment and interpretation of the spectrum of the unfolded state through the extensive knowledge of the spectrum of the folded state 2. These techniques have been applied to the study of a number of other proteins. In the case of one of these, staphylococcal nuclease, two distinct conformations of the folded form exist in equillbrium. One- and two-dimenslonal magnetization transfer experiments have demonstrated the interconversion of these two states with each other and with two unfolded states 3. Further, one dlmenslonal multlple saturation techniques have been used to explore the kinetics of the interconverslon of the different states, and to explore the re]atlve significance of different pathways of folding and unfolding. A structural basis for the observed behaviour has been proposed and is supported by the results of
slte-dlrected mutagenesls experlments 4. The experiments have therefore permitted specific dynamical events in the foldlng of a protein to be defined. I. 2. 3. 4. C.M. Dobson and P.A. Evans, Biochemistry 2_~3, 4267 (1984) C.M. Dobson, P.A. Evans and K.L. Willlamson, FEBS Letters 168, 331 (1984); C.M. Dobson, P.A. Evans, C. Redfleld and K.D. Topping, to be published. R.O. Fox, P.A. Evans and C.M. Dobson, Nature 320, 192 (1986) C.M. Dobson, P.A. Evans and R.0. Fox, to be publlshed.
Page 1 and 2: 28 th �� - 1987 Asilomar
Page 3 and 4: i 28tb Expcr menz t NhAR Cortfcrcnc
Page 5 and 6: Program for the 28th ENC April 5-0,
Page 7 and 8: Monday Evening 7:30 - 9:00 A. N. Ga
Page 9 and 10: 10:00 - 10:30 10:30 - 12:00 C. S. Y
Page 11 and 12: Sunday - PM TIME DOMAIN MAGNETIC RE
Page 13 and 14: Sunday - PM MF33 - POSTERS INCREASE
Page 15 and 16: Sunday - PM MF3 - POSTERS NMR SPECT
Page 17: Monday - AM Determination of Protei
Page 21 and 22: Monday - AM STRUCTURE AND DYNAMICS
Page 23 and 24: Monday - AM NOE STUDIES Philip H. B
Page 25 and 26: Monday - PM The NMR Gyro: An Applic
Page 27 and 28: Monday - PM NMR for the Detection o
Page 29 and 30: Tuesday - AM SELECTIVE DATA SAMPLIN
Page 31 and 32: Tuesday - AM INDIRECT DETECTION OF
Page 33 and 34: Tuesday - AM NOVEL APPROACHES TO TH
Page 35 and 36: Tuesday - PM SUPPRESSION OF DIPOLAR
Page 37 and 38: Tuesday - PM WF54 - POSTERS 2D EXCH
Page 39 and 40: Tuesday - PM WKI2- POSTERS QUANTITA
Page 41 and 42: Tuesday - PM WF64 - POSTERS FLOW IM
Page 43 and 44: . . . 4. -2- D. J. Singel, R. D. Ke
Page 45 and 46: Wednesday - AM THE DETERmiNATION OF
Page 47 and 48: Wednesday - AM Field Cycling NMR as
Page 49 and 50: Wednesday - AM Applications of Soli
Page 51 and 52: Notes
Page 53 and 54: Thursday - AM Gradient Transient Ef
Page 55 and 56: Thursday - AM PULSE SHAPING FOR TWO
Page 57 and 58: Thursday - AM ACTIVE SHIELD MAGNETS
Page 59 and 60: o u i.Z o Kiln - Poster Session Lay
Page 61 and 62: MF01 MF03 MF05 MF07 MF09 MFll MF13
Page 63 and 64: 1MF59 MF61 MF63 MF65 MF67 MF69 MF71
Page 65 and 66: 0.* Presenter MK43 Boyd, J. MK45 Ju
Page 67 and 68: MF3- POSTERS NMR SPECTROSCOPY BELOW
Page 69 and 70:
MF7 slV NMR: A NEW PROBE OF METAL I
Page 71 and 72:
MF11 TELLURI~-I~5 AND CADMIUM-Ill N
Page 73 and 74:
MF15 PHOSPHORUS POISONING OF THE AU
Page 75 and 76:
MFI9 AN EFFICIENT STATOR/ROTOR ASSE
Page 77 and 78:
MF23 - POSTERS NMR INVESTIGATIONS O
Page 79 and 80:
MF27 MULTINUCLEAR SOLID-STATE NMR S
Page 81 and 82:
MF31 SECOND OBSERVATION OF IH MASS
Page 83 and 84:
MF35 RELAXATION MECHANISMS AND EFFE
Page 85 and 86:
MF39 HIGH RESOLUTION DNP-NMR AND KN
Page 87 and 88:
MF43 DESIGN AND USE OF A PULSE SHAP
Page 89 and 90:
MF47 SOLID STATE NMR STUDIES OF ISO
Page 91 and 92:
! 1.7 MF51 ULTRA-HIGH RESOLUTION IN
Page 93 and 94:
MF55 DYNAMIC PARAMETERS FROM NONSEL
Page 95 and 96:
MF59 THE CONE COIL - AN RF GRADIENT
Page 97 and 98:
MF63 NON-INVASIVE SPIN LABELING OF
Page 99 and 100:
MF67 MAGNETIC RESONANCE IMAGING WIT
Page 101 and 102:
MF71 A DESIGN OF HOMOGENEOUS SELF-S
Page 103 and 104:
MF75 NMR IMAGING USING CIRCULAR SIG
Page 105 and 106:
MF79 IMAGING AND IN WVO SPECTROSCOP
Page 107 and 108:
MK3 OLIGONUCLEOTIDE STRUC'I13RE ~ B
Page 109 and 110:
MK7 IMPROVEMENTS OF THE 2D TRANSFER
Page 111 and 112:
MKI1 THE STRUCTURE OF THE SUBTILISI
Page 113 and 114:
MKI5 NA-23 NMR-INVISIBILITY IN LIVI
Page 115 and 116:
MKI9 SEQUENTIAL INDIVIDUAL 1H NMR R
Page 117 and 118:
MK23 MATER SUPPRESSION TECHNIQUES F
Page 119 and 120:
MK27 QUANTITATIUN OF 1-D SPECTRA WI
Page 121 and 122:
MK31 SYMMETRY RECOGNITION APPLIED T
Page 123 and 124:
MK35 NETVORKING AND AUTOMATION IN T
Page 125 and 126:
MK39 A Partial Excitation Method in
Page 127 and 128:
MK43 MjECHANIStCSOFCOHEREHCETRANSFE
Page 129 and 130:
MK47 AN IMPROVED CHORTLE PULSE SEQU
Page 131 and 132:
o °~ tO. o .9,o Kiln - Poster Sess
Page 133 and 134:
WF02 WF04 WF06 WF08 WF10 WF12 WF14
Page 135 and 136:
WF66 WF68 WF70 WF72 WF74 WF76 WF78
Page 137 and 138:
WK48 Presenter Rance, M. Title Impr
Page 139 and 140:
WF4 FIXED FIELD GRADIENT NMR DIFFUS
Page 141 and 142:
WF8 MULTIPLE ~ANTUH AND 129XE NMR S
Page 143 and 144:
WFI2 DETERMINATION OF ENZYME-STEROI
Page 145 and 146:
WF16 CHARACTERIZATION OF TRANSITION
Page 147 and 148:
WF20 DESIGN OF AN EFFICIENT PROBE F
Page 149 and 150:
WF24 VT CP/MAS NMR OF ANTIFERROMAGN
Page 151 and 152:
WF28 DYNAMIC NUCLEAR POLARIZATION I
Page 153 and 154:
WF32 SOLID STATE NMR OF PROTEINS P.
Page 155 and 156:
WF36 A PROTON MAS NMR METHOD FOR DE
Page 157 and 158:
WF40 NONAXIALLT SYMMETRIC DIPOLAR C
Page 159 and 160:
WF44 PROTON DETECTED SIP-IH HETCOR
Page 161 and 162:
WF4S SEGMENTAL DYNAMICS IN NYLON 66
Page 163 and 164:
WF52 ANALYSIS OF CONPLBX NIXTURES B
Page 165 and 166:
WF56 LOCALIZED PROTON DENSITY. RELA
Page 167 and 168:
WF60 SENSITIVITY OF SURFACE COILS T
Page 169 and 170:
WF64- POSTERS NMR FLOW IMAGING C. L
Page 171 and 172:
WF68 The SWIFT Method for In Vlvo L
Page 173 and 174:
WF72 THE DESCI~IPTII]N AND ELIMII~T
Page 175 and 176:
WF76 RAPID ~ F R A M E IMAGING Kenn
Page 177 and 178:
WF80 MULTIPLE QUANTUM FILTERING: US
Page 179 and 180:
WK4 P.E.COSY, DOUBLE QUANTUM FILTER
Page 181 and 182:
WK8 ISOTROPIC MIXING IN DNA Peter F
Page 183 and 184:
WK12 - POSTERS QUANTITATIVE INTERPR
Page 185 and 186:
WK16 MULTINUCLEAR SOLID STATE NMR S
Page 187 and 188:
WK20 ImQm~ss ~ M~/TIDIM~SIGNAL ~ C
Page 189 and 190:
WK24 BROADBAND HETERONUCLEAR DECOUP
Page 191 and 192:
WK28 SELECTION OF COHERENCE TRANSFE
Page 193 and 194:
WK32 C~4IC~L EXCHANGE IN METAL £X/
Page 195 and 196:
WK3C LINEAR PREDICTION Z-TRANSFORM
Page 197 and 198:
WK40 PULSE SHAPING FOR SOLVENT SUPP
Page 199 and 200:
WK44 TWO-DIMENSIONAL POMMIE 13C NMR
Page 201 and 202:
WK48 IMPROVED TECHNIQUES FOR THE AC
Page 203 and 204:
Ackerman, J. L ........... MK25 Ada
Page 205 and 206:
Lee, K.-B ................. WF2 Lei
Page 207 and 208:
Zciglcr, R ................. MF27 Z
Page 209 and 210:
Connie L. Ace Ethicon Inc. Route 22
Page 211 and 212:
Alan Benesi Penn State University D
Page 213 and 214:
Hark S. Brown Yale School of Hed. D
Page 215 and 216:
Michael Cooper Lockheed MSC 0/48-92
Page 217 and 218:
F. David Doty Doty Scientific Inc.
Page 219 and 220:
James S. Frye Colorado State Univ D
Page 221 and 222:
Herbert Gutowski University of Illi
Page 223 and 224:
Edward S. Hsi Union Carbide Corp. P
Page 225 and 226:
Rodney V. Kastrup Exxon Research &
Page 227 and 228:
Guang-Huei Lee Sun Refining/Hkting
Page 229 and 230:
John HcAutiffe Univ of Cincinnati O
Page 231 and 232:
Robin A. Nissan Naval Weapons Cente
Page 233 and 234:
Dattas L. Rabenstein Dept. of Chemi
Page 235 and 236:
Ronald Sager Quantum Design 11568 S
Page 237 and 238:
Robert Skarjune 3M Company Btdg 201
Page 239 and 240:
R. Tearson See program Ann N. Thaye
Page 241 and 242:
David E. Wemmer Univ of California
Page 243:
Notes
show all

th  - 1987 - 51st ENC Conference

Create successful ePaper yourself

Delete template?

Save as template?