th  - 1987 - 51st ENC Conference

MF31
SECOND OBSERVATION OF IH MASS NMR OF LIPIDS AND MEMBRANES;
AND 170 CROSS POLARIZATION OF INORGANIC SOLIDS
by
Eric O1dfleld,* John Bowers, Jeff Forbes ,. Cynthla Husted,
Thomas H. Walter and Xi Shan
University of llllnois at Urbana-Champalgn, 505 South Mathews Avenue,
Urbana, Illlnols 61801, USA
We have obtained very high resolution solid-state IH MASS NMR
spectra of unsonicated liquid crystalline lipid bilayers, and selected
biological membranes (e.g. rod outer sesments). Resolution is as good
as with sonlcated, single bllayer vesicles. T1s , linewidths, order
parameters and 2D experiments wlll be presented. A typical 2D (NOESY)
experiment on DMPC(I, 2-dimyrlstoyl-sn-glycero-B-phosphocholine) is shown
below, on the left:
• • 4' "q
"'° ~ °
]p, ,. ~,o
~:',. •
| 4 | | 1
PPM FROM TM$
Drug binding and other studies, will also be outlined.
--I
~mzo
We have also investigated 170 cross polarization in a variety of
inorganic solids. The type of resolution of overlapping second-order
quadrupolar broadened powder patterns achievable in complex inorganic
solids is illustrated by the static and CP results on talc shown above,
on the right.
-an
m