th  - 1987 - 51st ENC Conference

WF44
PROTON DETECTED SIP-IH HETCOR OF DNA FRAGMENTS
Robert Santini*
Claude R. Jones
David Gorenstein
Department of Chemistry
Purdue University
West Lafayette, IN 47907
Many spectrometers can be converted to simultaneous broad band operation
of both the transmit-receive and decoupler channels. With some extra equipment
(an additional broad band board, a PTS 160, filters, and a 10 watt amplifier) the
conversion of an XL-200A back and forth between a dual broad band configuration
and a standard configuration suitable for use in a multiuser environment can be
accomplished with a few cable changes. Proton detected sIp-~H HETCOR spectra
off synthetic DNA fragments with up to 14 base pairs were obtained in a dual
broad band mode using a standard 5rnm probe. The proton transitions were first
saturated (1) and a constant time sequence (2) was used. The pulse sequence
was phase cycled to give a pure absorption phase spectrum after a hypercomplex
transform.
1. V. Sklenar, H. Miyashiro, G. Zon, H.T. Miles, and A. Bax,
FEBS Letters (1986), 208, 94
2. H. Kessler, C. Griensinger, J. Zarbock, and H.R. Loosli, J.
Magn. Reson. (1984), 57, 331