th  - 1987 - 51st ENC Conference

MK21
ASPECTS OF PROTEIN STRUCTURE DETERMINATIONS WITH NMR
ERIK R,P, ZUIDERWEG*o DAVID G, NETTESHEIM AND
EDWARD T, OLEJNICZAK
NMR RESEARCH. ABBOTT LABORATORIES, ABBOTT PARK. ILLINOIS
60064
1H 2D NMR AND COMPUTER METHODS TO OBTAIN THE DATA NECESSARY
FOR THE DETERMINATION OF THE 3D STRUCTURE OF PEPTIDES AND
PROTEINS WILL BE DISCUSSED AND EXEMPLIFIED WITH RESULTS
OBTAINED ON RECOMBINANT CSA, A 75 AMINO ACID PROTEIN. ToPics
TOUCHED UPON WILL INCLUDE PHASE COHERENT DECOUPLING FOR
SOLVENT SuPPREsSION, ZZ SPECTROSCOPY FOR COMPLETE
"FINGERPRINTS", PRESATURATIONLESS HOHAHA SPECTROSCOPY OF
PEPTIDES IN H20 SOLUTION AND PHASE SENSITIVE COCONOSY FOR
AMIDE PROTON EXCHANGE STUDIES, SPECTRAL BASELINE DISTORTIONS
AND COMPUTER BASED CORRECTION METHODS WILL BE DISCUSSED.
STRATEGIES FOR NOE IDENTIFICATION IN CROWDED SPECTRA, SUCH AS
THE UTILIZATION OF SPIN DIFFUSION, WILL BE ILLUSTRATED. THE
USE OF THE DISMAN DISTANCE GEOMETRY PROGRAM BY W. BRAUN AND
N. Go FOR THE 3D STRUCTURE DETERMINATION OF THIS PROTEIN WILL
BE DESCRIBED AS WELL AS METHODOLOGY TO REFINE THE STRUCTURES
OBTAINED.