th  - 1987 - 51st ENC Conference

MK45
SIMULTANEOUSLY OBSERVING THE HOMONUCLEAR
AND HETERONUCLEAR EDITED SIGNALS WITHOUT
AN X NUCLEUS DECOUPLER
T. Jue
Dept. of Mol. Biophysics and Biochemistry
Yale University
New Haven, CT 06511
The promise of sensitivity enhancement has spurred research
efforts to edit the *H NMR spectrum. For in vivo studies, the
metabolic pathways have been followed with ,3C precursors, but
can be followed with enhanced sensitivy with |*sc}-*H editing
strategies. In particular, the one dimensional techniques based
on J modulation behavior have yielded successful results in
brain, liver, and muscle experiments. However, these extant
methods yield only the edited *3C-*H signals upon subtraction of
the alternate, modulated spin-echo experiments, but do not
necessarily give edited *2C-*H signals upon addition -- only the
overall *2C-*H resonances which are often encumbered with the
endogenous background lipid signals.
But in vivo studies require both the *2C-*H and *3C-*H
signals to probe the important question of specific activity,
which is posed by the various sources of a metabolic pool.
Consequently, we have devised a simple scheme to simultaneously
obtain both the homonuclear and heteronuclear edited signals
without a *sc decoupler. Pivotal metabolites are amenable to
such a winnowing scheme.