Journal of Film Preservation - FIAF

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Archives at Trebsín and Hradistko in a five-year study. The results
obtained in the Hradistko archive, where the storage conditions were
constant during the whole study period, show a low and constant air
contamination by fungi and bacteria. In contrast, the Trebsín archive
was at the start of the study (1984) in an entirely unsuitable state, and
the results indicate that the microbial contamination was 10 times
greater that in the well maintained archive. This is confirmation of a
direct relationship between storage conditions and microbial
contamination.
The incidence of micro-organisms on film cans was one thousand
times higher than in the airborne levels. The number of microorganisms
on the floor was practically identical to that on the cans. A
high density was found during a collection from wall surfaces (2-500
colonies on the dish), and the incidence of micromycetes in the archive
increased during the summer, in correlation with the situation outside
the archive building.
The quantitative analysis published by Opela identify 15 genera, the
most prevalent being, Aspergillus, 50% of the identifed fungi,
Cladosporium, 20% of the total, and Penicillium, another 20%. However,
numerous other types were found in insignificant levels. The types of
fungi found in Tresbín and Hradistko archives and on the film materials
are shown in Table 3.
Trebsín (Air) Hradistko (Air) On the film
Aspergillus versicolor (50%)* Aspergillus versicolor X
Cladosporium cladosporioides (20%) Cladosporium cladosporioides X
Penicillium frequentens (10%) Penicillium frequentens X
Penicillium lanosocoeruleum (10%) Penicillium lanosocoeruleum
Penicillium citrinum Penicillium citrinum
Aspergillus repens Aspergillus repens
Botrytis cinerea Botrytis cinerea
Trichoderma viridiae Trichoderma viridiae X
Mucor spp Mucor spp X
Rhizopus spp Rhizopus spp
Alternaria Alternaria
*Percentage of abundance from the total of isolated fungi
From the data, Aspergillus, Penicillium, Cladosporium, Tricoderma and
Mucor were located on the films, indicating their attraction to
materials present in the cinematographic film.
In the study, several conclusions were established.
53 Journal of Film Preservation / 67 / 2004
Penicillium roquefortii
Penicillium lanosum X
Table 3. Fungi identified in Tresbín and Hradistko archives 40 , isolated from air and films