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Program Book - Master Brewers Association of the Americas

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P-98<br />

Withdrawn<br />

P-99<br />

Antioxidant activities and phenolic pr<strong>of</strong>iles <strong>of</strong> millet wine<br />

during storage<br />

JING-IONG YANG (1), Chin-Yu Su (1)<br />

(1) National Kaohsiung Marine University, Taiwan<br />

Cereal wine has been claimed to have health-promoting effects,<br />

which may be related to antioxidant activity. This research presents<br />

information related to <strong>the</strong> flavonoid and total phenol contents <strong>of</strong><br />

millet wine stored for different periods (1, 4, 6, 8, or 10 months)<br />

at 4°C and <strong>the</strong>ir antioxidant activities, including linoleic acid<br />

peroxidation inhibition, reducing powder, ferrous ion chelating<br />

capacity, and scavenging activity on <strong>the</strong> free radical DPPH during<br />

storage compared with syn<strong>the</strong>tic antioxidants, such as α-tocopherol<br />

and BHA. Moreover, twelve phenolic compounds, including gallic<br />

acid, protocatechuic acid, gentisic acid, (+)-catechin, vanillic acid,<br />

caffeic acid, syingic acid, (–)-epicatechin, ρ-coumaric acid, ferulic<br />

acid, cinnamic acid, and quercetin in millet wine were identified<br />

by HPLC. This study aimed to compare <strong>the</strong> antioxidant activities <strong>of</strong><br />

millet wines stored during different periods and to analyze phenolic<br />

constituents for <strong>the</strong>ir contributions to antioxidant activities.<br />

Results indicated that millet wines exhibited noticeable antioxidant<br />

activities and that linoleic acid peroxidation inhibition reducing<br />

powder were highly correlated with total phenol contents. Moreover,<br />

DPPH free radical scavenging activity and reducing powder were<br />

highly correlated with flavonoid contents.<br />

Jing-Iong Yang has worked in <strong>the</strong> National Kaohsiung Marine<br />

University, Taiwan, since 2001. He is an associate pr<strong>of</strong>essor in <strong>the</strong><br />

Seafood Science Department <strong>of</strong> KMU. His current research focuses on<br />

<strong>the</strong> functionality <strong>of</strong> foods and beverages. Jing-Iong received his B.S.<br />

degree in agricultural chemistry and M.S. degree in food engineering<br />

from <strong>the</strong> National Taiwan University in 1991 and 1996, respectively.<br />

From 1996 to 2000 he studied at Karl J. Siebert’s laboratory<br />

(brewing chemistry major) at Cornell University and was awarded a<br />

Ph.D. degree in food science and technology.<br />

P-100<br />

The use <strong>of</strong> response surface methodology to optimize malting<br />

conditions <strong>of</strong> teff (Eragrostis tef L.) as a raw material for<br />

gluten-free foods<br />

MARTIN ZARNKOW (1), Cynthia Almaguer (2), Felix Burberg (1),<br />

Werner Back (1), Elke Arendt (3), Stefan Kreisz (4), Martina Gastl<br />

(1)<br />

(1) Lehrstuhl für Technologie der Brauerei I, Technische Universität<br />

München-Weihenstephan, Freising; (2) Jacob’s University Bremen,<br />

Bremen, Germany; (3) Department <strong>of</strong> Food and Nutritional Science,<br />

National University <strong>of</strong> Ireland, University College Cork, Ireland; (4)<br />

Novozymes AS, Bagsvaerd, Køpenhavn, Denmark<br />

Celiac disease (CD) is a condition in which <strong>the</strong> person’s body reacts<br />

to <strong>the</strong> prolamins <strong>of</strong> wheat, rye, barley, and oats. The only way to treat<br />

CD is a total lifelong avoidance <strong>of</strong> gluten consumption. In this study<br />

teff (Eragrostis tef L.), which belongs to <strong>the</strong> family Poaceae, which is<br />

regarded as gluten-free, was used as raw material. The objective <strong>of</strong><br />

this study was to optimize malting conditions to produce a glutenfree<br />

malt <strong>of</strong> high quality for gluten-free foods. Teff, with a thousand<br />

kernel weight <strong>of</strong> 0.3–0.4 g, needed special arrangements like small<br />

sieves etc. Teff has a remarkable agronomic advantage because<br />

its water requirement is probably <strong>the</strong> lowest <strong>of</strong> any major cereal.<br />

Response surface methodology was used to investigate <strong>the</strong> influence<br />

<strong>of</strong> three malting parameters, vegetation time, degree <strong>of</strong> steeping and<br />

temperature, on <strong>the</strong> quality <strong>of</strong> teff malt. Each predictor variable was<br />

tested at three levels. Vegetation times were 1, 2 and 3 days, degrees<br />

<strong>of</strong> steeping were 44, 50 and 52% and vegetation temperatures were<br />

18, 21 and 25°C. A kilning temperature <strong>of</strong> 65°C was used. The<br />

analyses used were based on methods outlined in EBC or by MEBAK.<br />

The raw material was yielded in 2006 in Utah, USA. A range <strong>of</strong> malt<br />

quality parameters was determined including extract, apparent<br />

attenuation limit, gelatinization temperature, α-amylase activity,<br />

β-amylase activity, limit dextrinase activity, Kolbach index, α-amino<br />

nitrogen, viscosity, and color. This publication shows clearly that<br />

on <strong>the</strong> one hand RSM is a proven method for testing <strong>the</strong> malting<br />

conditions <strong>of</strong> unknown cereals, and on <strong>the</strong> o<strong>the</strong>r hand Eragrostis tef<br />

is a crop with potential as a raw material for malting purposes.<br />

Martin Zarnkow apprenticed as a brewer and maltster from 1989<br />

to 1991 at a small brewery in Frankonia. Zarnkow finished a Dipl.-<br />

Ing. (FH) degree with an option in brewing technology in 1996 at<br />

TU München Weihenstephan. Zarnkow worked as a brew master<br />

for one year in a medium-sized brewery in Germany. Since 1997,<br />

Martin has been a scientific assistant and head <strong>of</strong> <strong>the</strong> beer laboratory<br />

at <strong>the</strong> Lehrstuhl für Technologie der Brauerei I at <strong>the</strong> TU München<br />

Weihenstephan. Since September 2005 Martin has been working as<br />

a Ph.D. research fellow at <strong>the</strong> University College <strong>of</strong> Cork, Ireland on<br />

“Malting and Brewing with Non-traditional Cereals.”<br />

115

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