Program Book - Master Brewers Association of the Americas

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O-43 The sensory directed product development of Presidente Light: A success story in the Dominican Republic ROY DESROCHERS (1), Joanna Borrell de Melo (2) (1) GEI Consultants, Inc., Woburn, MA; (2) Cerveceria Nacional Dominicana, Santo Domingo, Dominican Republic Developing a successful new beer has many challenges. This presentation will describe the sensory directed process Cerveceria Nacional Dominicana and GEI used to meet these challenges in the development of Presidente Light. The first step was to benchmark existing beer flavor in the Dominican market using flavor maps generated by professional sensory panelists. The next step was to understand consumer needs for beer flavor. A professional sensory panel selected a group of beers that represented a wide range of flavors to be used in the consumer test. The moderator for the consumer sessions was trained to use a unique facilitation approach to get participants to score overall liking, generate terms, identify key drivers of flavor, and rate the beers on their key drivers. In addition, participants were asked to score both their favorite beer and their ideal beer. The same beers assessed by consumers were also evaluated by a professional sensory panel using standard flavor attributes. The combination of consumer and professional scores for each sample allowed us to interpret consumer responses into actionable information. In addition, we were able to model consumer responses to predict overall liking for future beers, as well as optimize individual flavor characteristics. Next, we used the consumer and professional flavor data to identify distinct flavor segments on our market beer flavor map. We determined which existing beers fell into existing flavor segments by plotting them on the same map. We were then able to identify new flavor opportunities in segments with few, or no, existing beers. We were also able to estimate the potential size of each flavor segment by using consumption data collected in the consumer test. Last, the professional sensory panel was used to evaluate the flavor of a set of prototype beers, plot them on the flavor maps, and determine if they fell in flavor segments that were of interest. Confirmation consumer tests were then conducted, and the final beer was selected and launched under the brand name Presidente Light. Presidente Light quickly gained significant market share and in less than two years has obtained over 50% of the Dominican beer market. Roy Desrochers received a B.S. degree in chemistry and geology from Tufts University in Medford, MA. He began employment with Arthur D. Little, Inc. in June 1984 as a chemist and sensory panelist in their Food Industries Group. Over his 18 years at Arthur D. Little, he worked on numerous beer flavor projects for leading brewers around the world. After leaving Arthur D. Little, he managed his own sensory company, DesSense, Inc., for five years and now works for GEI Consultants, Inc. He has taught beer flavor at the MBAA Brewery Packaging Technology Course for over 24 years. In addition, he has conducted beer flavor workshops at the MBAA Annual Conventions in Austin, TX, and Milwaukee, WI, and at MBAA district meetings, including District New England, District Northwest, and District Caribbean. In addition to his support of MBAA activities, he is a member of the American Water Works Association (AWWA) Taste and Odor Committee and the American Society for Testing Materials (ASTM) Committee E18 – Sensory Methods. 86 O-44 New technology for gluten-free conventional brewed beers STEFAN MARX (1), Oliver Franz (1) (1) N-Zyme BioTec GmbH, Darmstadt, Germany Celiac disease is an auto-immune disease caused by certain proteins called prolamins (gluten) which are found in wheat and barley. The incidence rate of celiac disease in the Western population is approximately 1:300 and is the most common chronic intestinal disease. Gluten intolerance is not only a topic for celiac patients but more often for health-conscious people. Consequently celiac patients and gluten-sensitive people have to live on a gluten-free or gluten-restricted diet, and of course the consumption of all malt beer has to be avoided. As there is a demand for beer in these groups of patients, different beers from gluten-free raw materials (sorghum, rice) and pseudocereals (buckwheat) are found on the market. But these types of beers differ significantly in sensory impression. We can demonstrate a new technology that allows the gluten-free rendering of classically brewed all-malt beers using the enzyme transglutaminase. This enzyme catalyzes the specific agglomeration of gluten and similar protein fractions. The agglomerated gluten particles can be filtered, resulting in a bright, clear beer with a gluten-free status. There are different methods available for the analysis of gluten in foods, among them immunochemical diagnostic kits (sandwich-ELISA) are widely used. However, these test kits do not work properly in beer, as the brewing process by itself includes hydrolysis reactions of proteins which could not be detected by these systems. Many beers containing high amounts of gluten peptides were said to be gluten-free, which was a misleading conclusion. Therefore, a new competitive ELISA kit has been developed for the detection of gluten peptides in beer. The new technology, which is protected by intellectual property, enables the specific removal of gluten from conventional brewed beer. With the new competitive test, gluten peptides are no longer detectable, and gluten-free status can be declared. This procedure can be used for any type of beer. There are no limitations regarding the brewing process. Hazy products like cloudy wheat beer have not been possible up to now. The characteristic parameters of the beer will not be changed. The sensory impression will remain the same as there is no change in the flavor profile. There is no loss in foam stability and cling. National regulatory bodies should be consulted for specific declaration guidelines at an early stage of product development, and local celiac associations should be contacted as well. The technology provides new opportunities for the brewing and beverage industry in the fastgrowing gluten-free market. Stefan Marx studied biology at the University des Saarlandes and the University Stuttgart-Hohenheim and graduated with a degree in technical biochemistry in 1991. From 1991 to 1995 he worked on his doctoral thesis at ETH in Zürich, Switzerland, focusing on biochemical and physiological issues of plant metabolism, including cereals. After obtaining his Ph.D. thesis, he returned to Germany and built up a research group at RWTH in Aachen. His group worked on the development of biotechnological processes for the production of functional food ingredients, including aspects of the metabolism of intestinal bacteria. In 2001 he left academia and joined the small biotech company N-Zyme BioTec to strengthen its foodstuff activities. Since 2005 he has been managing director of the company, which supplies not only R&D services to the food industry, but also develops its own technologies and products.
Technical Session XIII: Microbiological Moderator: Dirk Bendiak, Molson-Coors Brewing Company, Etobicoke, ON, Canada Dirk Bendiak received his B.S. degree (Hon.) in genetics from the University of Alberta in 1975 and his Ph.D. degree in molecular biology from York University in 1980. He began work at Molson Breweries Canada Ltd. in 1981 as a senior microbiologist. In 1995 he began work as the corporate operations specialist for Molson Canada, delving into areas of brewing and packaging troubleshooting. As of June 1999, he has been back in the Brewing Department as the microbiology and brewing specialist, working on optimization of yeast propagation and yeast management practices, microbiological issues, CIP issues, malt issues, and brewhouse capability studies. In April 2000 he was the corporate brewer responsible for licensed brewing in Canada. In July 2001 his role changed to specialist, brewing and packaging quality, with responsibilities for licensed brewing, troubleshooting, and quality performance improvement. In April 2005 Dirk became as the manager, quality services, responsible for the analytical, sensory, and microbiology services for the corporate group. He is currently the manager of quality services in the Molson Toronto brewery. He is a member of the ASBC and is serving on the Technical Committee and has been program chair, subcommittee chair, and president. He is also a member of the MBAA, American Society of Microbiologists, and Canadian Genetics Society and an associate member of the Institute of Brewing. O-45 Development of detection medium for hard-to-culture beer spoilage lactic acid bacteria KOJI SUZUKI (1), Shizuka Asano (1), Tomoo Ogata (1), Yasushi Kitagawa (1) (1) Asahi Breweries, Ltd., Research Laboratories of Brewing Technology, Ibaraki, Japan Beer spoilage lactic acid bacteria (LAB) are generally difficult to detect using culture media. Among beer spoilage LAB, Lactobacillus paracollinoides and Lactobacillus lindneri strains are found in hardto-culture states upon primary isolation from brewery environments and often cause microbiological incidents without being detected by quality control tests in breweries. Nevertheless, detection media have often been evaluated with easy-to-culture beer spoilage LAB strains that are maintained in nutrient-rich laboratory media, an environment considerably different from those found in nature. This study therefore aimed to acquire hard-to-culture beer spoilage LAB as bioresources and develop a medium for detecting these groups of microorganisms. Four hard-to-culture beer spoilage LAB strains, belonging to L. paracollinoides and L. lindneri, were obtained by repeatedly subculturing the wild-type strains in beer. In contrast to the wild-type counterparts, these beer-adapted strains were found to be hardly detectable on MRS agar, a typical medium for detecting beer spoilage LAB in the brewing industry. To develop a countermeasure against these hard-to-culture beer spoilage LAB, a beer-based medium was modified. As a consequence, the supplementation of a small amount of MRS medium was found to enhance the growth of the hard-to-culture beer spoilage LAB strains obtained in this study. In addition, sodium acetate was shown to improve the selectivity of this beer-based medium. Further comparative study was performed with five other media widely used for the detection of beer spoilage LAB in the brewing industry. This experiment revealed that the newly developed medium, designated ABD medium, possessed superior sensitivity for hard-to-culture beer spoilage LAB and comparable sensitivity with easy-to-culture beer spoilage LAB. Moreover, ABD medium was found to suppress the growth of nonspoilage microorganisms and thereby allow the selective growth of beer spoilage LAB, a feature not observed with other detection media. These results suggest that the detection by ABD medium can be used as an indicator for differentiating the beer spoilage ability of LAB without additional confirmatory tests in breweries. Further field studies with ABD medium revealed that more than half of the test samples collected in this study contained hard-to-culture beer spoilage LAB, suggesting considerable proportions of beer spoilage LAB are in hard-to-culture states in beer and related environment. Taken together, ABD medium is considered an effective tool for comprehensive detection of beer spoilage LAB in breweries. Koji Suzuki received a M.S. degree in agricultural chemistry from Tokyo University, Japan. He joined Asahi Breweries, Ltd. in April 1992 as a microbiologist. Since April 2003, he has functioned as chief researcher in the Research Laboratories of Brewing Technology and principally studied beer spoilage microorganisms. He received a Ph.D. degree from Tokyo University in 2004 and an award from the Brewing Society of Japan in 2007 for his work concerning hop resistance to beer spoilage lactic acid bacteria. He is currently a vicechair of BCOJ Analysis Committee and also serves on the editorial board of the Journal of the Institute of Brewing. 87
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Program Book World Brewing Congress
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BECOPAD www.becopad.com Pure depth
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Table of Contents About Each Organi
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Your customerscan savorhand-crafted
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European Brewery Convention EBC, si
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PALATINOSE from BENEO-Palatinit is
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BREW HOUSE VESSELS/RENOVATION PROCE
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CONSERVING WATER HAS A RIPPLE EFFEC
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Thank you to the following WBC 2008
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Schedule at a Glance HCC denotes Ha
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Program HCC denotes Hawaii Conventi
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Pre-congress Course: Topics in Brew
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Opening Plenary Session Keynote and
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Daily Schedule — Monday, August 4
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Pairings workshop. The effect of gl
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2:30 p.m. O-35. A survey and explan
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Page 39 and 40: 10:05 - 11:50 a.m. Technical Sessio
Page 41 and 42: Post-congress Course: EPR Plus: Pra
Page 43 and 44: Poster Session HCC Kamehameha Hall
Page 45 and 46: P-126 The use of carbon dioxide in
Page 47: P-186 The making of a professional
Page 50 and 51: I-3 ERAB engaging in putting knowle
Page 52 and 53: IBD Symposium: It’s Education, St
Page 54 and 55: I-11 Control of flavor production i
Page 56 and 57: Organization The BCOJ was establish
Page 58 and 59: Miller, he spent 25 years with Carg
Page 60 and 61: Workshop Biographies W-1 To Ferment
Page 62 and 63: Cindy-Lou Dull received a B.S. degr
Page 64 and 65: W-8 Packaging: Draft Beer from Rack
Page 66 and 67: Technical Session Abstracts & Biogr
Page 68 and 69: Technical Session II: World Class M
Page 70 and 71: Technical Session III: Stability Mo
Page 72 and 73: O-12 Investigations on the behavior
Page 74 and 75: Technical Session V: Finishing Mode
Page 76 and 77: O-20 Changes in protein and amino a
Page 78 and 79: Technical Session VII: Cereals/Pseu
Page 80 and 81: O-28 Psychophysical models for the
Page 82 and 83: O-32 Influence of harvest date, gro
Page 84 and 85: O-35 A survey and explanation for t
Page 86 and 87: O-39 High cell density fermentation
Page 90 and 91: O-46 Rapid detection and identifica
Page 92 and 93: O-50 Improvement of premature yeast
Page 94 and 95: O-54 Comparison of different wort b
Page 96 and 97: O-58 Gene expression analysis of la
Page 98 and 99: O-62 Bioconversion of brewer’s sp
Page 100 and 101: Poster Session Abstracts & Biograph
Page 102 and 103: P-68 Mycotoxin lateral flow assays
Page 104 and 105: P-72 Evaluation of optical O 2 meas
Page 106 and 107: P-76 Quantitatively identifying PYF
Page 108 and 109: Award from the ASBC in 2005 with Ma
Page 110 and 111: Poster Session: Brewhouse Moderator
Page 112 and 113: P-88 Wort boiling by batch rectific
Page 114 and 115: P-92 Prediction of malt sugar conte
Page 116 and 117: P-96 The use of response surface me
Page 118 and 119: P-101 The use of response surface m
Page 120 and 121: P-104 Understanding the value gener
Page 122 and 123: P-108 The relationship between wate
Page 124 and 125: P-113 Visual recording of process c
Page 126 and 127: P-117 Practical usages of electroly
Page 128 and 129: P-121 Improved plant cleanliness, p
Page 130 and 131: P-125 Advances in preparation and p
Page 132 and 133: P-130 Fermentation course predictio
Page 134 and 135: discussed in this presentation. We
Page 136 and 137: P-139 Effective use of yeast nutrie
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P-143 Aspects of beer quality and e
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P-147 An innovative regenerable fil
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P-151 Citra—A new special aroma h
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Poster Session: Malt Moderator: Kel
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P-158 New barley varieties and thei
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P-162 Effect of high temperature-hi
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Poster Session: Nutrition Moderator
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P-170 Controlling fills in the brew
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candidate and research associate at
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P-179 Improved operating conditions
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Poster Session: Premature Yeast Flo
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P-187 The effect of hop harvest dat
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P-191 Sensory detection thresholds
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P-195 A new approach to sensory eva
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their maturation capacity and to sa
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P-203 Anaerobic and aerobic beer ag
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Poster Session: Yeast Moderator: Gr
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P-214 Functional analysis of mitoch
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P-218 Detection of yeast in brewery
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Bring the Congress Home on CD! WBC
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Maca, W., W-1 Maeda, H., P-71 Maeda
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WBC 2008 Exhibits Representatives f
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180 %alcohol. Included in the range
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111 Hop Breeding Company LLC, 31 N.
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115 PureMalt Products Ltd., Victori
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Exhibit Numeric Listing 104 Oregon
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Advertisers’ Index A. Ziemann Gmb
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