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Program Book - Master Brewers Association of the Americas

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P-156<br />

Pulverized wort for brewing compared to traditional products<br />

MIRJAM HAENSEL (1), Jens Voigt (1), Sabine Grüner (2), Andreas<br />

Kilzer (3), Karl Sommer (1), Eckhard Weidner (3)<br />

(1) Technische Universität München, Lehrstuhl für Maschinen-<br />

und Apparatekunde, Wissenschaftszentrum Weihenstephan,<br />

Freising; (2) Adalbert-Raps-Zentrum Weihenstephan, Freising;<br />

(3) Ruhr-Universität Bochum, Lehrstuhl für Verfahrenstechnische<br />

Transportprozesse, Fakultät Maschinenbau, Bochum<br />

German beer is famous and popular worldwide. However German<br />

ingredients are not available all over <strong>the</strong> world and are difficult and<br />

expensive to transfer. Moreover <strong>the</strong> procedure according to <strong>the</strong><br />

German purity law demands sufficient experience in mashing and<br />

appropriate raw materials. Today it is possible and common to apply<br />

malt extract, which is extracted from wort by two-stage vacuum<br />

evaporation, instead <strong>of</strong> pure malt. Unhopped, thickened wort<br />

normally contains up to 30% water. The use <strong>of</strong> drying procedures<br />

(spray drying, freeze drying) enables <strong>the</strong> production <strong>of</strong> malt extract<br />

powder as it is already known for hop and yeast products. The lower<br />

weight and enhanced microbial stability <strong>of</strong> <strong>the</strong>se powders are <strong>the</strong><br />

main advantages in transport costs, shelf life and handling compared<br />

with liquid products. For <strong>the</strong> production <strong>of</strong> beer from malt extract,<br />

powder is diluted with water and adjusted to <strong>the</strong> desired gravity. In<br />

this work several different worts were brewed using four different<br />

malt products in order to compare <strong>the</strong> quality <strong>of</strong> <strong>the</strong> resulting<br />

beer. One beer was made in a traditional manner with pure malt,<br />

according to <strong>the</strong> German purity law. Ano<strong>the</strong>r beer was made with<br />

conventional liquid malt extract. The o<strong>the</strong>r two beers were brewed<br />

with two innovative powders. One powder consists <strong>of</strong> silicic acid<br />

which is normally used as a filtration additive and malt extract. The<br />

second powder consists <strong>of</strong> pure malt extract produced by a special<br />

drying process using supercritical carbon dioxide. Brewing was<br />

performed with <strong>the</strong> same process parameters in <strong>the</strong> brewhouse,<br />

during fermentation and storage. In order to compare <strong>the</strong> different<br />

beers, a set <strong>of</strong> common analysis and sensory tests were made.<br />

Mirjam Haensel graduated as a brewing engineer in 2006. Her<br />

diploma <strong>the</strong>sis at <strong>the</strong> Technische Universität München, Lehrstuhl für<br />

Technologie der Brauerei I, Wissenschaftszentrum Weihenstephan,<br />

Freising, was concerned with specification <strong>of</strong> buckwheat and<br />

o<strong>the</strong>r pseudocereals in beer. Since September 2007 she has been<br />

working as a Ph.D. student and research assistant at <strong>the</strong> Technische<br />

Universität München, Lehrstuhl für Maschinen- und Apparatekunde,<br />

Wissenschaftszentrum Weihenstephan, Freising. Her Ph.D. studies<br />

deal with powdering <strong>of</strong> malt extract.<br />

P-157<br />

Characteristics <strong>of</strong> oxalate oxidase in <strong>the</strong> malt<br />

MAKOTO KANAUCHI (1), Charles Bamforth (2)<br />

(1) Department <strong>of</strong> Food Management, Miyagi University, Sendai,<br />

Japan; (2) Department <strong>of</strong> Food Science and Technology, University<br />

<strong>of</strong> California, Davis, CA<br />

Although oxalate has long been recognized as a problem in beer,<br />

<strong>the</strong>re have been few studies devoted to <strong>the</strong> understanding <strong>of</strong> factors<br />

that impact its levels in beer. As part <strong>of</strong> our investigations on this<br />

topic, we have located and begun to study an enzyme from malt<br />

that can remove oxalate, namely oxalate oxidase. Oxalate oxidase<br />

is located in <strong>the</strong> aleurone layer and increases in activity during<br />

malting. It has been purified by ion exchange and size-exclusion<br />

chromatography. The molecular weight <strong>of</strong> <strong>the</strong> enzyme is 58.500.<br />

It has an optimum pH <strong>of</strong> 4.0. The Km for oxalate is 0.1 mM and<br />

for oxygen is 0.46 mM. The enzyme is activated by zinc and flavan<br />

adenine dinucleotide.<br />

Makoto Kanauchi graduated from Tokyo University <strong>of</strong> Agriculture,<br />

Japan, in March 1996. He received a Ph.D. degree in bio-regulation<br />

control from Tokyo University <strong>of</strong> Agriculture in March 1999. He<br />

worked in Pr<strong>of</strong>essor Charlie Bamforth’s Laboratory in Food Science<br />

and Technology, University <strong>of</strong> California at Davis, CA (1999–2003).<br />

Subsequently, he was employed at <strong>the</strong> Institute <strong>of</strong> Food Science in<br />

Fuji Oil Corporation in Moriya, Ibaraki, Japan, as a researcher<br />

(2003–2005). Since April 2005, he has been an assistant pr<strong>of</strong>essor<br />

in <strong>the</strong> Department <strong>of</strong> Food Management, Miyagi University. He has<br />

also been a lecturer in enzymology and alcoholic beverages (mainly<br />

spirits and wine) at <strong>the</strong> Tokyo University <strong>of</strong> Agriculture since October<br />

2005.<br />

143

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