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Program Book - Master Brewers Association of the Americas

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P-189<br />

Influence <strong>of</strong> non-volatile beer constituents on <strong>the</strong> bitter taste<br />

perception <strong>of</strong> iso-α-acids<br />

STEFAN HANKE (1), Werner Back (1), Martina Gastl (1)<br />

(1) Lehrstuhl fuer Technologie der Brauerei I, Freising, Germany<br />

The hop derived bitter taste is one <strong>of</strong> <strong>the</strong> most important and<br />

most intrinsic properties <strong>of</strong> beer. The main bittering sources are<br />

<strong>the</strong> iso-α-acids and in <strong>the</strong> case <strong>of</strong> <strong>the</strong> use <strong>of</strong> downstream products<br />

derivates <strong>of</strong> <strong>the</strong> iso-α-acids, which are responsible for most <strong>of</strong> <strong>the</strong><br />

perceived bitterness. In addition to <strong>the</strong> concentration <strong>of</strong> <strong>the</strong> bitter<br />

principles <strong>the</strong>re are fur<strong>the</strong>r factors influencing <strong>the</strong> perception <strong>of</strong><br />

beer bitterness. Because <strong>of</strong> <strong>the</strong> complex mixture <strong>of</strong> ingredients<br />

beer can possibly mask some taste and flavor compounds. Masking<br />

is a widespread phenomenon and a typical effect in heterogeneous<br />

mixtures, like beer. There are different <strong>the</strong>ories about <strong>the</strong><br />

contribution <strong>of</strong> ethanol, dextrins and pH-value on <strong>the</strong> bitter taste<br />

perception <strong>of</strong> beer. The perception <strong>of</strong> bitterness and <strong>the</strong> harmony<br />

<strong>of</strong> all ingredients is assumed to have a great contribution on <strong>the</strong><br />

drinkability <strong>of</strong> beer. Drinkability means a specific harmony <strong>of</strong><br />

all antagonistic substances in <strong>the</strong> beverage. It is influenced by<br />

technological and non-technological (physiological) parameters.<br />

One <strong>of</strong> <strong>the</strong>se technological parameters is <strong>the</strong> composition <strong>of</strong> <strong>the</strong><br />

beverage. Changing <strong>the</strong> composition <strong>of</strong> a beverage has a great effect<br />

on <strong>the</strong> perception <strong>of</strong> bitterness. In our experiments we changed<br />

<strong>the</strong> composition <strong>of</strong> model solutions and <strong>of</strong> an unhopped lager. In<br />

aqueous model solutions dextrins had masking properties and<br />

increased <strong>the</strong> threshold <strong>of</strong> iso-α-acids. In an unhopped beer <strong>the</strong>y<br />

showed <strong>the</strong> same pattern. Dextrins may act as masking agents in <strong>the</strong><br />

beverage and are an antagonistic part <strong>of</strong> <strong>the</strong> bitter taste. Ethanol has<br />

for some tasters a bitter taste. This results in a decreased perception<br />

<strong>of</strong> bitter acids in <strong>the</strong> beer, so <strong>the</strong> thresholds increase. But it seems<br />

that <strong>the</strong> bitter taste <strong>of</strong> ethanol itself is reduced by o<strong>the</strong>r constituents<br />

in <strong>the</strong> beer. So we claim that ethanol, dextrins and <strong>the</strong> acidity <strong>of</strong> a<br />

beverage have a great impact on bitter taste perception. A harmonic<br />

composition <strong>of</strong> <strong>the</strong>se factors is essential for beverages with a high<br />

drinkability.<br />

Stefan Hanke was born in 1980. From November 1999 to July<br />

2004 he studied brewing science and beverage technology at<br />

Munich Technical University (Weihenstephan), graduating as an<br />

engineer with a Dipl.-Ing. degree. During his studies he worked for<br />

and received practical training at different German brewing and<br />

malting companies. Since September 2004 he has been a scientific<br />

employee at <strong>the</strong> Lehrstuhl fuer Technologie der Brauerei I, Freising-<br />

Weihenstephan, Germany (Pr<strong>of</strong>essor Back). From December 2006<br />

until May 2007 he headed <strong>the</strong> institute’s Small Scale and Pilot Scale<br />

Brewery Department. Since May 2007 he has been responsible for <strong>the</strong><br />

HPLC and GC Laboratory <strong>of</strong> <strong>the</strong> institute. His main research topics<br />

are <strong>the</strong> influence <strong>of</strong> hops on beer drinkability, as well as <strong>the</strong> influence<br />

<strong>of</strong> beer matrix on bitter taste.<br />

P-190<br />

Identification <strong>of</strong> aroma compounds associated with sourness<br />

KEIKO ISHIKAWA (1), Masato Kawasaki (1), Takeo Imai (1),<br />

Yutaka Ogawa (1)<br />

(1) Kirin Brewery Co., Ltd., Yokohama-shi, Japan<br />

Beer aroma is comprised <strong>of</strong> various compounds, <strong>the</strong> balance <strong>of</strong><br />

which is very important to taste pr<strong>of</strong>ile. Consumer research reveals<br />

that indications <strong>of</strong> sourness can decrease taste preferences. It is<br />

conceivable that <strong>the</strong> sour aroma in aroma components may be a<br />

contributing factor, separately from sour flavors represented by<br />

organic and inorganic acids. Depending on <strong>the</strong> concentration<br />

levels, this sour aroma may be characterized as odorant. To improve<br />

beer flavor requires that <strong>the</strong> aroma associated with this sour odor<br />

be controlled. In our research, we utilized GC-olfactometry in<br />

an attempt to identify compounds associated with sour odors in<br />

beer. As a result <strong>of</strong> our research, we were able to detect at least 10<br />

components linked to sour odors, mainly odors associated with fruit,<br />

cheese or natto. Using GCMS, we were able to identify aliphatic<br />

acids, aliphatic acid esters, and higher alcohols. For several <strong>of</strong> <strong>the</strong>se<br />

compounds, we were able to confirm an identical aroma between<br />

a reference standard and our GC-olfactometry. These compounds,<br />

ei<strong>the</strong>r individually or in combination, may contribute to sourness in<br />

beer.<br />

Keiko Ishikawa graduated from <strong>the</strong> Department <strong>of</strong> Food Science<br />

(dietician course) <strong>of</strong> <strong>the</strong> Otsuma Women’s University, Faculty <strong>of</strong><br />

Home Economics, in 2002. She joined <strong>the</strong> Kirin Brewery Co., Ltd.<br />

Research Laboratories for Brewing that same year and, for <strong>the</strong> next<br />

four years, worked in beer instrumental analysis. During 2006, she<br />

started work in brewing technology development, and at present, she<br />

is engaged in microanalysis, focused mainly on odor substances.<br />

159

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