Letno poročilo 2005

sevov mikroorganizmov, predvsem ekstremofilnih
gliv in jo redno dopolnjujemo z novimi
izolati.
BIBLIOGRAFIJA
18 izvirnih znanstvenih ~lankov
1 kratki znanstveni prispevek
1 samostojni znanstveni sestavek v
monografiji
3 objavljeni znanstveni prispevki na
konferencah
23 objavljenih povzetkov znanstvenih
prispevkov na konferencah
1 patentna prijava
1 patent
5 predavanj na tujih univerzah
1 prispevek na konferenci brez natisa
8 diplom
1 doktorat
GLAVNI DOSE@KI V LETU 2005
V letu 2005 smo v sodelovanju z drugimi
laboratoriji na Kemijskem in{titutu in zunanjimi
uporabniki pridobili preto~ni citometer s
sorterjem in CD spektrometer, dve pomembni
aparaturi za raziskave v molekularni in strukturni
biologiji.
V zvezi z raziskavami receptorjev LPS oz.
inhibicijo signalizacije Toll-u podobnih receptorjev
(TLR) smo v lanskem letu napravili
pomemben korak naprej, saj smo s pomo~jo
fluorescen~nih prob pokazali na pomen
hidrofobnih interakcij pri prepoznavanju in na
tej osnovi testirali vezavo vrste spojin, za katere
je bilo prej `e ugotovljeno, da inhibirajo celi~no
signalizacijo preko NF-kB. Vlogo hidrofobnih
interakcij v vezavnem `epu smo potrdili tudi z
usmerjenimi mutantami MD-2, ki je vezan na
izvenceli~no domeno TLR4. Vezavo enakih spojin
kot na MD-2 smo analizirali tudi na homologa
Der p2 in GM2-AP in ugotovili, da je GM2-AP
najbr` bolj primeren strukturni model za MD-
2.
Poleg inhibicije receptorja lahko nevtralizacijo
delovanja endotoksina dose`emo tudi s
spojinami, ki se direktno ve`ejo na LPS in
Laboratorij za biotehnologijo
Laboratory of Biotechnology
lection, with more than 3,000 different strains,
with emphasis on extremophiles, which is regularly
expanded by new species isolated from
their natural environment.
BIBLIOGRAPHY
18 Original Scientific Articles
1 Review Article
1 Independent Scientific Component Part
in a Monograph
3 Published Scientific Conference Contributions
23 Published Scientific Conference Contribution
Abstracts
1 Patent Application
1 Patent
5 Invited Lectures at Foreign Universities
1 Unpublished Conference Contribution
8 Undergraduate Theses
1 Doctoral Dissertation
IMPORTANT ACHIEVEMENTS IN 2005
In 2005 a flow cytometer with cell sorting ability
and a CD spectrometer were purchased with
the help of other Laboratories of the National
Institute of Chemistry as well as other users.
The new equipment is very important for research
in molecular and structural biology.
In the research field on LPS receptors or inhibition
of signalization by Toll-like receptors (TLR)
an important step forward was achieved. With
the help of fluorescent probes we succeeded
to prove the importance of hydrophobic interactions
in recognition of LPS and on this basis
we tested binding of a number of compounds,
previously known to inhibit cell signalization via
NF-kB. The role of hydrophobic interactions in
the binding pocket was confirmed also with
point mutations of MD2, which is associated
to the extracellular domain of TLR4. The binding
of the same compounds as on MD2 was
also analyzed on the homologues Der p2 and
GM2-AP and found that GM2-AP is probably a
more suitable structural model for MD2.
In addition to receptor inhibiton it is possible
to neutralize the endotoxin activity also by com-
143